Distribution Of Helicobacter Pylori And Metaplastic Lesion In Gastric Mucosa

AIM: To study the relationship between the distribution of H. pylori(Hp) infection and metaplastic changes in the gastric mucosa.METHODS: Whole-mucosal step sectioning studies were performed using 10 surgically resected stomach specimens (5 cases of gastric or duodenal ulcers. 5 of gastric carcinoma) that histologically showed Hp infection. Serial paraffin sections were made from every tissue and and stained as follows: (1)hematoxylin and eosin stain, (2) immunostaining for Hp, and (3) double-staining using human gastric mucin (M1) immunostaining and alcian blue (AB). After each section was histologically evaluated, the positively stained areas were plotted on photographic copies showing the stomach specimens.RESULTS: The staining properties were as follows: (1) HP~-. (2) complete intestinal metaplasia (IM) , (3) incomplete intestinal metaplasia, and (4) pseudopyloric gland metaplasia and mucous neck cells (PM) . The 10 stomach specimens were then classified into three types: In type â… , the distributions of Hp and the metaplastic changes, especially PM, overlapped. In types â…¡ and â…¢, the distributions of Hp and the metaplastic changes, especially IM, did not overlap. In 4 out of 5 type â…  cases, Hp was observed in areas of incomplete IM epithelia. Type â…¡ and â…¢ cases generally showed higher degrees of mucosal inflammation and atrophy of proper glands than the type â…  cases. Four out of 5 type â…¡ and â…¢ cases were associated with carcinoma, whereas only 1 type â…  case was associated with carcinoma. The other cases were associated with ulcer lesions.