Role Of BMP2 In Early Retinopathy Of Diabetic Rat Model

Objective: Study BMP2 expression in early retinopathy of diabetic rats with or without aminoguanidine(AG) treatment.Method: Total of 45 rats (species) were selected randomly for this experiment. A group of 8 were used for normal control(no diabetic inducement). The remaining 37 rats were treated with a single dose intraperitoneal (i。p。) injection of streptozotocin(stz). Out of the stz treated rats, 30 rats successfully developed the diabetic symptoms. These 30 rats were then evenly divided into 3 groups. The first group rats received no treatment(positive control), whereas the second group rats received i。p。injection of AG (50mg/kg) once per day for consecutively 9 weeks and the last group received i.p. injection of fresh fruit of monordica charantial .On week ten, all rats were sacrificed. Their intact retina were extracted and processed in preparation of paraffin sectioning. In order to analyze the BMP2 expression level among the 3 groups (normal control, positive control and the AG treated), an immunohistochemistry experiment (SABC) were conducted side by side using the paraffin section samples prepared from the 3 groups. The result were analyzed by the imaging software (Lecia Q win V3)Result: Immunohistochemical staining showed the BMP2 expressionlevel in rat retina was significantly lower in diabetic positive controlgroup than that in AG treated group. The BMP2 expression level in bothnormal control group and AG treated group is prominently higher ascompared to that in positive control group.Conclusion: BMP2 as a negative growth regulator plays important role inmediation of retinopathy in diabetic rats. Measurement of BMP2expression level may provide a new clinical reference for diabeticretinopathy treatment prognosis. Objective: to investgate BMP7 and TGF-B expression in early retinopathy of diabetic rats with or without aminoguanidine(AG) treament.Method : Total of 35 rats (species) were selected randomly for this experiment. A group of 8 were used for normal control(no diabetic inducement). The remaining 27 rats were treated with a single dose intraperitoneal (i.p.) injection of streptozotocin(stz). Out of the stz treated rats, 21 rats successfully developed the diabetic symptoms. These 21 rats then evenly distributed into 2 groups. The first group rats rece ived no treatment(positive control), whereas the second group rats received i.p. injection of AG (50mg/kg) once per day for consecutively 9 weeks On week ten, all rats were sacrificed. Their intact retina were extracted and processed in preparation of paraffin sectioning. In order to analyze the BMP7 and BMP7 and TGF-βexpression level among the 3 groups (normal control, positive control and the AG treated), an immunohistochemistry experiment (SABC) were group.and compared to the BMP7, the TGFβexpression level is in opp conducted side by side using the paraffin section samples prepared from the 3 groups. The result were analyzed by the imaging software (Lecia Q win V3).Result: Immunohistochemical staining showed the BMP7 expression level in rat retina was significantly lower in diabetic normal control group than that in positive group. The positive group is lower than the AG treated osite direction. The normal control group is the highst ,and The positive group is higher than the AG treated group.Conclusion: BMP7 and TGF-βplay an important role in mediation of retinopathy in diabetic rats. Measurement of BMP7and TGF-B expression level may provide a new clinical reference for diabetic retinopathy treatment prognosis.