| ObjectiveTo study the expression of Podocalyxin in renal pathological gradesof Henoch-schonlein purpura nephritis (HSPN),and explore the role ofthe Podocalyxin expression in kidney and injury of podocyte during theprogressive course of HSPN.MethodsThe expression of Podocalyxin in 4 normal controls and 56pathological grades of HSPN(Ⅱa,Ⅱb,Ⅲa,Ⅲb,Ⅳa,Ⅳb,Ⅴa andⅤb)before treatment in kidney were detected by immunofluorescence stainingand quantitatively analyzed by image analysis system. In the meantime,the expression of podocyte Podocalyxin in 8 normal controls and 56pathological grades of HSPN in urine were detected too.Results1.The expression of Podocalyxin of normal control, HSPNⅡa andⅡb was linar and continuous along glomerular capillary loops in kidney,and no significant defference of the percent and the level of theimmunofluorescence staining area between them(P＞0.05).2. The expression of Podocalyxin of HSPNⅢa,Ⅲb,Ⅳa,Ⅳb,Ⅴa andⅤb was linar and granular along glomerular capillary loops partially inkidney,while no expression was detected in the crescentic or scleroticarea. The image analysis showed a gradual decrease in the expression intensity of Podocalyxin from grade HSPNⅡb toⅤb in kidney(P＜0.01).3.Podocyte marked by anti-podocalyxin antibody was found in urinefrom grade HSPNⅢtoⅤ.Conclusion1.The expression change of Podocalyxin in kidney decreased graduallyfrom grade HSPNⅡb toⅤb, which might play an important roleduring the progressive course of HSPN.2.The podocyte Podocalyxin detected in urine from grade HSPNⅢtoⅤ, it may hint that the podocyte was injuried in HSPN. |
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