Study The Effect Of Jiaweishengjiangsan On Th1/Th2 In The Serum Of Henoch-Schonlein-Purpura Nephritis (HSPN) Mice

Objective: Study the effect of Jiaweishengjiangsan on Th1/Th2 in the serum of Henoch-Schonlein purpura nephritis(HSPN) mice to investigate the anti-HSPN immunological mechanism of Jiaweishengjiangsan.Methods: 72 SPF level KM mice are divided into 6 groups randomly with 12 cases in each group.6 groups are named normal control group(N group)、model group( M group) 、 Tripterysium Glycosides group( L group) 、Jiaweishengjiangsan high dose group(G group)、Jiaweishengjiangsan middle dose group(Z group)、Jiaweishengjiangsan low dose group(D group) respectively.Mouse models are created with the method of taking gliadin orally and injected Indian ink by caudal vein, meanwhile all drugs are adiministered by intragastric administration everyday.After 17 weeks, the index of serum creatinine 、 24 hours urinary protein excretion and Urine red blood cell number are detected to assess if the model is created successfully.Measure IFN-γ、IL-4 levels in each group which will reflect Th1/Th2 with the method of ELISA.Results:The mouse model was created successfully.We find that every treatment group has raised IFN-γ level and reduced IL-4 level compared with the M group.The difference has statistical significance.The most effective group is Z group.Conclusion:Jiaweishengjiangsan can treat HSPN by reversing Th1、Th2 levels.