| Exercise induced fatigue is body can't keep its physiological process on an order level or can't keep on certain sports strength. To study the mechanism of exercise induced fatigue and medical health care support improve the exercise ability and fatigue resume has a very important theory, practice, society benefit for improving exercise achievement and keeping fit. Take the data of [Ca2+]i in myocardial cells, plasma biochemical indices, the apparent of heart, ECG and myocardium mechanics to explore the effect of exercise induced fatigue on rats'heart function. And then study the anti-exercise fatigue function of ginsengnoside Rb1, which can scavenge oxygen free radicals, improve the activity of SOD, block cell calcium channel opening.The exercise-induced fatigue models in rats were established by 15 days chronic swimming training. Using the automatic biochemical analysis instruments to take the data of plasma biochemical indices; take the standardⅡ-lead ECG; take the data of myocardium mechanics in vivo and vitro; and using the ion imaging technique to measure [Ca2+]i in mycardial cells.Exercise-induced fatigue made the CK of rats blood plasma increased from 357.83±50.45IU/L to 558.1±101.2IU/L (P<0.01), increased 55.97%; Glu increased from 7.63±1.62mmol/L to 10.28±2.08mmol/L (P<0.05), increased 34.67%; Ca2+ decreased from 2.57±0.05mmol/L to 2.21±0.11mmol/L(P<0.01), decreased 13.9%. Exercise-induced fatigue made rats'heart increased obviously and myocardial hypertrophy. The weight of rats'heart increased from 0.573±0.03g to 1.049±0.08 (P<0.01), increased 82.89%. Exercise-induced fatigue made rats'ECG R wave increased rapidly from 0.5012±0.05mv to 0.9072±0.3152mv(P<0.01), increased 81.01%. Exercise-induced fatigue made rats'myocardium mechanics much higher than control. In vivo, exercise- induced fatigue, LVSP increased from 15.88±0.47kPa to 18.14±0.19kPa (P<0.01), increased 14.24%; +dp/dt increased obviously (P<0.05), increased 45.32%. In vitro, LVSP in Exercise-induced fatigue(5.681±0.497kPa) was much higher than control (4.539±0.389kPa, P<0.01), 25.17% higher; +dp/dt increased obviously (P<0.05) in exercise-induced fatigue rats, increased 30.78%. The [Ca2+]i in fatigue myocardial cells (308.58±69.48nM) are much higher than control (107.77±27.04nM).Ginsengnoside Rb1 can make the CK rise of exercise-induced fatigue rats blood plasma decrease to 292±63.89IU/L(P<0.01), decreased 47.68%; Rb1 can make Glu increased more to 14.85±3.73mmol/L(P<0.05), increased 44.46%; Rb1 can make it recover to 2.47±0.08mmol/L (P<0.01), increased 11.61%. Exercise-induced fatigue can reduce blood plasma Ca2+ concentration, enhance in-cell Ca2+ concentration, and Rb1 can increase blood plasma Ca2+ concentration, decrease in-cell Ca2+ concentration, delay cell calcium overload. Rb1 did little to decrease R wave in exercise-induced fatigue rats. Ginsengnoside Rb1 decreased myocardium mechanicals in fatigue rats, and protect cardiac muscle. In vivo, exercise- induced fatigue, Rb1 recovered LVSP to 16.41±0.43kPa(P<0.01), decreased 9.53%; Rb1 also recovered +dp/dt(P<0.01), decreased 40.34%. In vitro, Rb1 can reduce LVSP rapidly to 2.737±0.595kPa (P<0.01), reduced 51.83%; Rb1 decreased +dp/dt rapidly too(P<0.01), decreased 46.53%. Rb1 also has the negative myocardium mechanics in control, but not significant to fatigue. Varapamil has similar effect on fatigue hearts, and more effective, but it reduced heart rate notably.Exercise-induced fatigue rats have higher myocardium mechanics, bigger heart, and get myocardial hypertrophy. Exercise-induced fatigue can reduce blood plasma Ca2+ concentration, enhance in-cell Ca2+ concentration in myocardial cells. Rb1 can increase blood plasma Ca2+ concentration, decrease in-cell Ca2+ concentration, delay cell calcium overload. Rb1 can decrease the myocardium mechanics of exercise-induced fatigue rats and play a positive role on cardiac protective, it shows fewer negative affection compare with Varapamil. |
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