The Effect Of Health Promoter-â…  (HP-â… ) Supplement On Improvement Of Exercise Performance And Mitigation Of Exercise-induced Fatigue

Objective: Health-promoting agent I composed of Bacopa monniera, Silybum marianumGaerth, Withania somnifera, Curcuma longa and Camellia sinensis was used for theintervention of mice. After intervention, physiological and biochemical parameters includingblood lactate (Bla), serum urea (BUN) level, malondialdehyde (MDA) content, serumsuperoxide dismutase (SOD) activity and glycogen content of the mice were determined.Meanwhile, the effect of HP1intervention on the improvement of exercise performance andmitigation of exercise-induced fatigue was evaluated. Furthermore, corresponding molecularmechanisms of HP1intervention on exercise performance enhancement and fatigue recoverywere further explored, which will provide a theoretical basis for the development ofnutritional supplements.Methods: Totally60male mice were randomly divided into four groups including sedentarycontrol group (C), exercise control group (CE), HP1intervention group (HP1) and HP1intervention coupled with exercise group (HP1-E). After adaptive feeding for one week, themice from CE group and HP1-E group were subjected to swimming training for10min on thefirst day, and then the swimming training with increment time of10min per day until60min.The whole experimental duration was8weeks. The mice from HP1and HP1E groups wereadministered with HP1at10:00am every day at the dose of2.5mg/d HP1dissolved in0.6mL of distilled water. After the HP1administration was more than2h, the mice wereprovided swimming training for60min every day. On the last day of the experiment, the micewere subjected to loaded swimming (5%body weight) to exhaustion. The loaded swimmingtime was recorded. The blood was harvested from eyeball immediately and the mice werethen sacrificed. The blood lactate (Bla), serum urea (BUN), serum superoxide dismutase(SOD), malondialdehyde (MDA) and glycogen content were also evaluated. Furthermore, thepossible signal pathways were explored by Western blotting to explore correspondingmolecular mechanisms. Results: After administration of HP-1for8weeks, the exhaustive swimming time of the micefrom CE, HP1and HP1-E groups revealed a significant prolonged when compared with thatfrom the sedentary group C (P <0.01); in addition, the HP1intervention could result in asignificant reduction of BUN, obvious increase of SOD activity and MDA level andimprovement of hepatic glycogen when compared with those in the control group (P <0.01).On the other hand, HP1intervention could induce the significant reduction in seruminflammatory cytokines such as IL-1β and IL-6.Conclusion: Health promoter I (HP-1) can improve exercise capacity, and delay the exercisefatigue through improving glycogen reserves, suppressing the occurrence of inflammation,enhancing antioxidant enzyme activities, and reducing lipid peroxidation. Therefore, thishealth promoter has promising prospects for the improvement of exercise capacity andmitigation of exercise-induced fatigue.