| OBJECTION To investigate the anti-fatigue effects and the mechanism ofsmall molecular peptides CMS 008 and CMS 001 on Balb/c mice, and the effect on CFS of small peptide CMS001.METHODS 1. According to the anti-fatigue test, the male Balb/c mice were tested for the exhaustive swimming time, liver starch, urea nitrogen and lactic acid in the blood serum. 2. To research the ultrastructure especially mitochondria and sarcoplasmic reticulum of the fatigued mice and the molecular peptide's effect, electron microscope was used to discover the ultrastructure difference between peptide group and saline group. 3. The MDA, SOD, AST, ALT levels in the blood serum were also detected to find the effects of CMS 008and CMS 001 on the exhaustive mice. 4. The effects of CMS008 and CMS001 on T lymphocyte transformation in vitro were tested to reflect their immune function. 5. Wheel System to Count Mice Activity was designed to count the mice's daily activity. 6. CP 0.1ml(6.0X 109 /ml) was intraperitoneally injected to female C57BL/6 mice to induce immunologically mediated fatigue. What we do is to investigate if CMS 001 had some therapeutic effect on CFS mice.RESULTS 1. CMS 008 can remarkably prolong exhaustive swimming time (p<0.05). CMS 001 high and low dose groups also can prolong exhaustive swimming time of mice. 2. All the CMS 008 and CMS001 groups can decline the BUN significantly after 90min swimming (p<0.05). 3. CMS 008 high and low dose groups enhance liver starch level significantly contrast to saline group (p<0.05). CMS001 high dose group can enhance liver starch (p<0.05), but the low dose group not. 4. As to lactic acid level in blood serum, it increased after acute short time exercise. All of the experiment groups can inhibit the produce of lactic acid and decrease the increased lactic acid (p<0.05). 5. Electron microscope gave adirect damage impression of acute long exercise. Skeletal muscle and myocardium's mitochondria and sarcoplasmic reticulum of saline group were damaged by piles and piles of myelin, calcium-overload can also be detected. Mitochondria turned into many vacuoles, sarcoplasmic reticulum lysised. CMS 008 group damaged little at the same time, and pathological changes were minimal to adapt to exercise. 6. After acute exercise, MDA and SOD gained positive findings statistically for the two peptides can decrease MDA and increase SOD contrast to saline group; After exhaustive exercise, AST and ALT levels in the blood were higher than the normal control group, but CMS 008(500ug/kg/d) group was relatively lower than saline group (p<0.05). CMS 001 (20ug/kg/d) group can also decreased AST and ALT levels of blood serum compared with saline group(P< 0.05). 7. CMS 001 can inhibit T lymphocyte transformation^ 0.001), but CMS008 hasn't the immune function.8. Wheel System to Count Mice Activity can count mice's daily activity. 9. Through CP injection, the CFS model mice declined their daily exercise below to 50% of their baseline, and the model control group kept their normal activity, CMS 001 and saline control's activity dropped radically to 20% of the normal activity, and the two groups were in the same percentage (p>0.05).CONCLUTIONS 1. CMS 008, CMS 001 have anti-fatigue effects on Balb/c mice. 2. Inhabiting protein lysis, protecting skeletal muscle and myocardium, cleaning out free radicals contribute to anti-fatigue of the small peptides. 3. Wheel System to Count Mice Activity can sensitively and precisely counts daily activity of mice, CMS 001 has little therapeutic effect on CFS. |
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