Establishment And Evaluation On Acute Myocardial Infarct Model With Kidney-yang Deficiency In Rats

Most of animal experiments still adopt etio-copying-patho animal model in present pharmacology research on traditional chinese drugs, paying much attention to illness, regardless of syndrome. Since it is difficult to reflect therapeutic principle and characteristic of determinating the treatment based on differentiation of symptoms and signs through animal model. And up to now there is no model to give consideration to illness and syndrome all over the world. It is a key technical platform to establish repeatable and operatable animal model combining illness and syndrome together. Now acute myocardial infarction model in rats is applied to evaluate coronary heart disease treating drugs, ignoring syndrome such as kidney-yang deficiency,as is viewed in coronary heart disease.For this reason we establish acute myocardial infarction in kidney–yang deficiency rats through ligating the left anterior descending coronary antery for 24 hours then subcutaneous injecting hydrocortisone.We observe general body condition, body temperature,body weight,food ingested and water ingested variation for 8 days during establish model ,and record immune organ-thymus index and spleen index at the end of the experiment.we evaluate kidney–yang deficiency model through comparison of above between normal group and kidney-yang deficiency group.We examine myocardium morphology- myocardial infarction scope(MIS)through NBT(nitrobenzene thiocyanate)staining; examine 1st,3st,5st min, maxal platelet facking fraction and platelet adhesion ratio, thrombo-length,thrombo-wet weight,thrombo-dry weight of thrombusformation in vitro,r,k and ma of thrombelasto graph, hematocrit(HCT)and blood sedimentation rate ( BSR ) with LiPuSheng hemorheology instrument;examine heart rate(HR),surface electrocardiogram-ST(Ⅱ),T(Ⅱ),ST(Ⅴ5)和T(Ⅴ5), systolic blood pressure(SBP),diastolic blood pressure(DBP),mean arterial blood pressure(MAP),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP) and rate of rise of left ventricular pressure(±dp/dtmax)with RM-6000 polygraph ; examine blood serum creatine kinase(CK) , aspartate aminotransferase(AST) and lactate dehydrogenase(LDH)activity by COBAS-FARA automatic biochemistry ; examine blood serum malondialdehyde(MDA),superoxide dismutase(SOD), glutathione peroxidase(GSH-Px),nitrogen monoxidum(NO)and free fatty acid(FFA) content by biochemistry kit,examine blood plasma prostaglandin I2(PGI2), thromboxan A2(TXA2),endothelin(ET),tumor necrosis factor(TNF-α)and atrial natriuretic peptide(ANP)by radio-immunity kit.Two model rats were treated with western medicine(Nitroglycerin injection)and traditional Chinese medicine(KuDieZi injection).we evaluate correlation bewteen acute myocardial infarction under kidney–yang deficiency and simple acute myocardial infarction.we can conclude:All animals in kidney-yang deficiency group decrease in activity,response slowly, dispirit disastrously, have lusterless fur, decrease in body temperature,body weight,food ingested,water ingested,thymus index and spleen index and so kidney-yang deficiency syndrome on,compared with normal group. Accordingly, acute myocardial infarction under kidney–yang deficiency model has been established successfully.All animals in acute myocardial infarction under kidney–yang deficiency model group(AMIKD)increase slightly in MIS compared with simple acute myocardial infarction group(AMI). Diversity has no statistical significance.It makes clear that acute myocardial infarction under kidney–yang deficiency does not aggravate simple acute myocardial infarction MIS.All animals in AMIKD increase slightly in platelet fackingfraction,platelet adhesion ratio thrombo-length,thrombo-wetweight,thrombo-dry weight,ma,HCTandBSR,increase slightly in r and k,compared with AMI. Diversity has no statistical significance.It makes clear that acute myocardial infarction under kidney–yang deficiency does not aggravate simple acute myocardial infarction hemorheology abnormality.HR,surface electrocardiogram-ST(Ⅱ),T(Ⅱ),ST(Ⅴ5)和T(Ⅴ5), SBP,DBP,MAP,LVSP,LVEDP and±dp/dtmax variation diversity has no statistical significance betweenAMIKD and AMI. It make clear that acute myocardial infarction under kidney–yang deficiency does not aggravate simple acute myocardial infarction left ventricle function abnormality.Blood serum CK,LDH,AST,MDA,SOD,GSH-Px,NO and FFA content variation diversity has no statistical significance betweenAMIKD and AMI. It make clear that acute myocardial infarction under kidney–yang deficiency does not influence obviously simple acute myocardial infarction left above blood serum substance content.Blood plasma PGI2,.TXA2,ET,TNF-αand ANP content variation diversity has no statistical significance betweenAMIKD and AMI. It makes clear that acute myocardial infarction under kidney–yang deficiency doesnot influence obviously simple acute myocardial infarction left above blood plasma substance content.Above index variation diversity has no statistical significance between acute myocardial infarction under kidney–yang deficiency treated with western medicine(Nitroglycerin injection)group(AMIKD&NG)and acute myocardial infarction under kidney–yang deficiency treated with traditional Chinese medicine(KuDieZi injection)group(AMIKD&KDZ).It makes clear that treating AMIKD and AMI with Nitroglycerin injection and KuDieZi injection has no statistical significant diversity.Our research paves the way to evaluate traditional pharmacological animal models scientificly and study for traditional Chinese drug and materials.