| Why immune methode is carried out so widely and efficiently in diagnose, prevention,lies in specificity of immune reaction and immune response. Specificity of antigen and antibody means complement of different material as one vital factor in immune response and reaction,which is proved by different experience. Regard to safety of traditional diagnosis , modern molecular biology are adopted to produce recombination protein, on one hand, it is safe and operational to express protein for antigen determined . on other hand, gene engineering is available to get a large amount of target protein. therefore, it is more easy to get more protein satisfied need of research .Breast cancer is the most prevalent cancer among women in the world, Characterization of tumor markers, as that of proteins highly expressed in malignant cells, is of prime importance in understanding the mechanisms underlying cancer initiation and progression.Mucins are high molecular weight glycoproteins that provide a protective layer on epithelial surfaces,MUC1 is expressed at the apical surface of ductal epithelia of tissues, including breast, pancreas, airway, and the gastrointestinal tract, where its functions include lubrication, protection and Signal transduction of the epithelia.type1 transmembrane mucin-like protein that contains a large extracellular domain, primarily consisting of a 20-amino acid repeat motif enriched in serine, threonine, and proline residues as well as a transmembrane domain and a 72-amino acid cytoplasmic tail, Alternative mRNA splicing generates a secreted form of MUC1, MUC1/SEC, lacking the transmembrane and cytoplasmic tail. Although MUC1/Y is a transmembrane protein that shares identical transmembrane and cytoplasmic domains with the MUC1/REP protein, it contains neither the repeat array domain nor its flanking region and, hence, is devoid of the hallmark mucin-like features. it was reported that the MUC1/Y protein is expressed by various human secretory epithelial tumors, but is not detectable in the adjacent normal tissue . Based on research, pET32a as fusion express vector is one of pET system, besides,Cp10 is also fusion partner ,both are aimed at expression and purification and identification efficiently, polyclonal antibody immunized hare is high titer, histochemisty result proves antibody against MUC1/Y can detect MUC1 and its isotype in breast tumor. So more researches are further to be carried out.Foot-and-mouth disease virus (FMDV) is the cause of a highly contagious disease of cattle and other cloven-hoofed animals (Brown, 1999). Foot-and-mouth disease (FMD) is the most economically significant animal viral disease worldwide. Mortality can be high in young animals in unvaccinated herds. As discussed previously, interaction of the RGD triplet on VP1 with cell integrins is considered a requirement for viral entry. It has been recently shown that the RGD triplet and surrounding amino acid residues contained in the G-H loop can become dispensable for FMDV growth in cell culture. By using Cpn10-VP1 fusion protein, establishing an indirect ELISA method can detect the specific antibodies against foot-and-mouth disease virus (FMDV) with no-viral antigen, and extend detection other populations by testing samples of pig. Therefore, Indirect ELISA is not only to detect animals infected FMDV but also provide reference as result of predict foot-mouth disease epidemic and reflection vaccine for immune response .To extend indirect ELISA, ELISA kit replaces virus with fusion protein as antigen coated to surveillance FMD.
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