Experimental And Clinical Study Of Orthotopic Liver Transplantation

Objective(1) To study the key surgical technique of orthotopic liver transplantation(OLT) in Chinese inbred miniature porcine and try to find out the way of the establishment of stable animal model. This process also can be surgical training for clinical practice as the same time.(2) To study the donor liver modification, recipient operation and postoperation management of clinical OLT.Methods(1) OLT were performed without veno-venous bypass(VVB) in Chinese inbred miniature porcine. The whole process including four parts: donor animal operation, donor liver modification, recipient animal operation and perioperation management. Induced anesthesia was given to all animals and after endotracheal intubation, anesthesia was maintained by combined intravenous medicine in recipients. Donor animal was exsanguinated blood for recipients via an abdominal aorta cannula. The donor liver was protected by double perfusion from abdominal aorta and portal vein. During donor liver modification, the diaphragm was reserved about 3mm around suprahepatic vena cava and was sutured by non-trauma seam in order to avoid blood leak and keep enough tenacity after anastomosis of vena cava. Recipients were...