Association Between Clara Cell Protein 10 And Airway Insufflation Pressures In Guinea Pig Asthma Models

Study objectives: To investigate the effect of peritoneal injected glucocorticoids on the levels of Clara cell protein 10 (CC10) in serum and lung tissue of a guinea pig model of asthma, and the relationship between the levels of CC10 and airway reactivity in a guinea pig model of asthma.Menthods: The 27 healthy guinea pigs are randomly divided into three groups: A normal control group, an allergic asthma group and a dexamethasone (DXM) pretreatment group, in wich a guinea pig asthma model was established by sensitization and challenge with ovalumin (OVA). Determine airway insufflation pressures (AIPs) of all groups with two-way electrophysiolograph, make inferior lobs of right lungs in three groups stain hematoxylin eosine (HE) to observe the change of lung tissue among three groups, and the CC10 levels in serum and lung homogenate were repeatedly determined by enzyme-linked immunosorbent assay (ELISA). Then get the relationship between the levels of CC10 and AIPs.Results: After the provocation of OVA, the AIPs of the allergic asthma group were significantly raised up than that of control and DXM pretreatment groups (P<0.05). The CC10 levels in serum of three groups were not different (P>0.05). The CC10 levels in lung homogenate of the allergic asthma group were significantly decreased than that of control and DXM pretreatment groups (P<0.05). The AIPs were significantly associated with the CC10 levels in lung homogenate (r= —0.528, P= 0.001), and had not statistically significant association with the CC10 levels in serum (P>0.05).Conclusions: The CC10 levels in lung homogenate was decreased, and had negative correlation with AIPs. We propose that CC10 takes part in the onset of asthma, and works as an important endogenous anti-inflammatory factor, and glucocorticoids may have an anti-inflammatory effect on the pathogenesy of asthma by increasing the CC10 levels of lung tissue.