| Background: Dental caries is one of the common diseases which is effected by multiple factors and taken on dental hard tissue Chronic destruction. Fluoride could prevent dental caries which is one of the maximum dedication to human beings of oral preventive medicine in 20 Centuries. Many techniques about fluoride preventing dental caries are being widespread applied now and have made enormous social effect and economic returns being recommended by WHO and oral authority institutions. But when their roles of preventing dental caries being confirmed, we found many problems, especially high concentrations fluoride could lead to fluoride-resistant strain selective growth when long-term local operated, fluoride-resistant strain would evidently increase bacterial plague's ability to cause dental caries in normal fluorine Concentrations; Next it could result in urgent and Chronic fluoride poisoning together with dental fluorosis; Moreover there were many problems about local operating fluoride, such as worse binding ability with dental face, transient remaining time in oral cavity, no target to cariogenic bacteria, and no convenient usage for patients. Fluor protector is one of the fluorides and there are two different concentrations (0.1% and 0.5%). Fluor protector had been operated on preventing dental caries in local areas, especially concentrated on epidemiology. And there are no correlative reports about laboratory research papers, so it is essential to investigate the utility of fluor protector about resisting dental caries systematically. Objective: We explored the utility in preventing dental caries about decreasing enamel Solubility, promoting enamel remineralization and inhibiting cariogenic bacteria growth based on predecessors' research, then compared the preventing dental caries effect of two different concentrations fluor protectors and provided reliable theory to disseminate fluor protector for population to prevent dental caries. Materials and Methods: 1. Growth suppression of two different concentrations fluor protectors to Streptococcus mutans and Streptococcus sobrinus. Streptococcus mutans and Streptococcus sobrinus suspensions were cultured anaerobicaly in BHI for respectively with the application of the same volume of 0.5% fluor protector, 0.1%fluor protector, 0.1%Chlorhexidine diacetate, 0.05% Sodium fluoride and 0.9% Saline. Then the CFU was counted. The growth suppression effects were compared to cariogenic streptococci. 2. Adhesiveness suppression of two different concentrations fluor protectors to Streptococcus mutans and Streptococcus sobrinus. Streptococcus mutans and Streptococcus sobrinus suspensions were cultured anaerobicaly in BHI for respectively with the application of the same volume of 0.5%fluor protector, 0.1%fluor protector, 0.05% Sodium fluoride and deionized water. Then the mass was measured by the means of capillaries. The adhesiveness inhibitory effects were compared to cariogenic streptococci. 3. Study of fluorine release of two different fluor protectors in vitro. A thin coating on one side of a polyester sheet(20mm×40mm)with fluor protector, then was painted each sheet of polyester with the fluor protector was placed coating into a polystyrene vial and added 20ml of artificial saliva, and the fluor protector coating became completely immersed in the solution of homeothermia, 370C at1,3,7,31,55,124 and 213 hours after immersion.The fresh solution replaced the older one for fluoride determination with a fluoride-specific ion electrode and a acidometer meter. The release condition was compared in vitro. 4. Study on inhibitory effect of two different concentrations fluor protectors on demineralization of human enamel. 40 non-carious human enamels were randomly divided into four groups with 10 samples in each group, of which 5 samples were used for experiment, 5 samples were used as spare ones. 0.5% fluor protector and 0.1% fluor protector were applied to the experiment groups, while 0.02% Sodium fluoride and deionizer water were applied to the control groups. Then th... |
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