Study On Re-mineralization Of Fluor Protector And Lanthanum Chloride On The Artificial Root Caries

Objectives:To study effects of Fluor protector and Lanthanum chloride used alone and jointly to deal with human root caries to supply experiment and reference in clinical application.Methods:Fifty non-caries extracted human anterior teeth and premolars were collected with dental crown removed and fenestration district in the tooth root face. After the artificial caries were formed by de-mineralization, the tooth were randomly divided into five groups with ten teeth each. Group A: Fluor protector-0.1% Lanthanum chloride treatment; Group B: Fluor protector treatment; Group C: 1000mg / L sodium fluoride treatment; Group D: 0.1% Lanthanum chloride treatment; Group E: de-ionized water treatment. Group C is for the positive control group, and group E is the negative. Each group was carried out re-mineralization cycle, proceed in the pH cycle models at 37 degrees oscillation to circulate, six times a day, eight days together. We made manufacture chips after re-mineralization circular process and analyzed the effects of re-mineralization by Laser scanning confocal microscope (LSCM) while observed lesions situation by Scanning electronic microscope (SEM).Results:1. Observed by SEM, at the fenestration district of teeth windowing in group A,a large number of mineral deposits were visible, with surface flat, structure compact well-distributed, and sediment was a little patch and streak; in group C mineral deposits were patch, streak, more uniform but there remain pores or cavities; the appearance of fenestration district of group B ranged between the group A and group C; in group D there were mineral deposits, with surface rough, and sediment were streak, granular, and loose, with existence of pores or cavities; in group E there were Pit-like defects in the surface roughness defect, and there were no obvious mineral deposits .2. Observed by LSCM, we saw the differences of fluorescence band before and after the cycle. The lesion depth and fluorescence intensity of each group enhanced after the cycle. Group E was the most obvious group, followed by group D, group C, group B, and group A. TF value of group E had a significant difference compared with other groups (P<0.05); TF value of group C had a statistically difference compared with other groups (P<0.05); but AREA value and AF value of group A and group B had no significant difference (P>0.05).Conclusions:1. Fluor protector and lanthanum chloride used alone and jointly can promote re-mineralization of root caries, and the effect of joint application was better than the two used alone.2. The effect of re-mineralization of root caries by Fluor protector was better than the 0.1% lanthanum chloride.