Comparation Of Transcetaneous Electrical Stimulation And Ultrashort Wave On Cerebral Ischemia Reprefusion In Rats

OBJECTIVECerebrovascular disease is common illness causing death and disablity. With investigation of cerebral arterial thrombosis deeply, we find the blood vessel come to repeifusion injury after vessel is recanalization in cerebral ischemia and blood flow restore recanalization after thrombolysis.It is proved that fastigial nucleus stimulation can increase local cerebral blood flow of cerebral cortex, deflate cerebral infarction volume, and improve limbs function recovery. But the treatment do not change brain tissue sugar metabolic rate. Fastigial nucleus stimulation could decrease TUNEL dying cell obviously and has neuroprotective effect. Negative heat effect of ultrashort wave therapy is grit increase between ion, pair ion and corpuscle in tissue. The Negative heat effect is investigated to lessen cerebral ischemia, to lighten cellogenic and vasogenic brain edema, and to conservancy in reperfusion injury.Focal cerebral ischemia in Wistar rats is produced by 2 hours of occlusion of the middle cerebral artery (MCAO) followed by 24 hours of reperfusion. The neurological deficits evaluated by Zea - Longa Scale. The rats are then subject to transcetaneous electrical stimulation treatment or ultrashort wave treatment on their head at 18 hours after the end of reperfusion. The cerebral water content is derermined by wet/dry weighing method and the infarct volume measured by 2, 3,5- triphenyltetrazolium chloride staining technique. The activity of bcl - 2 and bax in ischemia tissue of brain and morphologic changes are tested.METHODSThere are 65 majority healthy Wistar rats used to MCAO with Zea - Longa method. The rats are then subject to transcetaneous electrical stimulation treatment or ultrashort wave treatment on their head at 18 hours after the end of reperfusion. The control group has not treatment. The rats are decapitated to recipe brain at 24 hours after the end of reperfusion.The brain is derermined by wet/dry weighing method and the infarct volume. The activity of bcl - 2 and bax in ischemia tissue of brain are tested. We use the T - test analysis the sample.RESULTS1. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in wet/dry weighing method. Transcetaneous electrical stimulation and ultrashort wave can diminish wet/dry weighing method. There is not statistics difference between them.2. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in the infarct volume. Transcetaneous electrical stimulation and ultrashort wave can diminish the infarct volume. There is not statistics difference between them.3. There is statistics difference between control group, transcetaneous electrical stimulation group and ultrashort wave group in the activity of bcl - 2 and bax in ischemia tissue of brain. Transcetaneous electrical stimulation and ultra-short wave can diminish apoptosis. There is not statistics difference between them.CONCLUSIONTranscetaneous electrical stimulation and ultrashort wave can decrease wet/ dry weighing method and the infarct volume, suppressor apoptosis in region is-...