Effects Of Using Ultrashort Wave In Early Period On Brain After Focal Ischemia Reperfusion In Rats

PrefaceAt present, cerebraovascular disease(CBVD) can be managed as the life-threaten-ing emergency that it has higher incidence, disability and mortality. Although we havedone acute diagnose to CBVD, only thrombolysis is taken asone of effectiv etherapiesafter stroke which might effective get over neuro function. An increasing number ofpeople are realizing that it will decrease disability and mortality of CBVD by usingphysical therapy. The study aims at observating whether ultrashort wave therapymightdecrease brain edema in the early period or not, infarct volume, the reponse of tumornecrosis factor-α(TNF-α)after the middle cerebral artery occlusion and reperfusion andproviding theory evidence clinically by utilizing modified Zea-Longa's method toduplicate the rat model.Materials and MethodsThere are 53 majority healthy Wister rats who unlimited male female used toMCAO with Zea-Longa method. The entering standard is the rat model is graded 2points by 5 levels grade made by Zea-Longa, i.e.rats turn around to another side offocus when walking. 53 rats are randomly divided into control group(untreated afterischemia reperfusion n=16), ultrashort wave treatment groupl(treated after ischemiareperfusion 6h n=16) and ultrashort wave treatment group2(treated after ischemiareperfusion 9h n=16), sham operation group (n=5). The rats are decapitated to recipebrain at 24h after the end of reperfusion. The hydrocephalus content and infarct volumeare weighed individually. The activity of TNF-αin ischemia tissue of brain are tested.The results are given as the mean±SD((?)±S). Groups are carried out by t test.Results1. There is not statistics difference between control group and ultrashort wave treatment group1, group2 in hydrocephalus content weighing method(p＞0.05).2. There is not statistics difference between control group and ultrashort wavetreatment group1, group2 in infarct volume weighing method(p＞0.05).3. Ultrashort wave treatment group1, group2 have higher gray values of TNF-αthan control group. There is statistics difference between control group and ultrashortwave treatment group1, group2(p＜0.05).ConclusionsThe trail find that it is not effective that the therapy of ultrashort wave to the injuryof cerebral ischemia and reperfusion. To some extent, it can increase gray values ofTNF-αwhich indiates pathologic injuries stronger than control group. But the varietyof infarct volume and hydrocephalus content is not significant.