| [Background and objective]The incidence and mortality of lung cancer is increasing rapidly and it severely endangers human health and life .The mechanisms of the carcinogenesis of lung tissue is very complex. Researches have showed that the process of carcinogenesis includes both transformation of certain genes and angiogenesis. Tumor angiogenesis is closely related with the changes in the function or structure of certain genes as well as positive and negative modulation of many cytokines.Cyclooxygenase-2(COX-2) is the key enzyme involved in prostaglandin production in pathologic satates such as inflamamatory processes and cancer. Researches show that COX-2 have profound effects on carcinogenesis through many ways, among which the major one is the enhancement of angiogenesis. The mutation of P53 gene is one of the most important events in the tumorigenesis of lung. The mutant type of P53 not only loses the function as tumor suppressor gene but also increase the expression of VEGF, thus can promote angiogenesis. There have been reports of cooperation of COX-2 and P53 in ovarian carcinoma and esophageal carcinoma ,but reports concerns COX-2 and its relation with P53 in lung cancer are rather rare. Our research employ immunohistochemical method to explore the expression of COX-2, P53 and MVD as well as the association between them.Our researches aims to provide theory basis for diagnosis and treatment of lung cancer.[Materials and methods]85 surgically resected non-small cell lung carcinoma tissues were collected. Among them male 55, female 30; of them, 45 cases were Squamous cancer, 40 cases were adenocarcinoma; In addition,of them, 16 cases were well-differentiated squamous cancer, 14 cases were moderately-differentiated squmaous cancer, 15 cases were poorly-differentiated squmaous cancer; 17 cases were well-differentiated adenocarcinoma, 23 cases were poorly-differentiated adenocarcinoma; and there were lymph nodes metastasis in 50 cases,and no lymph nodes metastasis in 35 cases; 20 cases of benign lung lesions were collected as control groups. All the tissues were fixed in 10% neutral formalin and embedded in paraffin. SP immunohistochemical method was performed to detect the expression of COX-2, P53 and MVD. The data were analyzed by software SPSS 10.0, Chi-square Test , and exact probability method were used to compare the difference of COX-2 and P53 expression between groups, Mest was used to compare the difference of MVD between groups.linear correlation was used to compare the difference between COX-2, P53 and MVD.a<0.05 was considered as level of tests.[Results]1 , The positive staining of COX-2 mainly located in cytoplasm. The rate of COX-2 expression is 5.0% in benign lung lesions. The positive expression was 56.5% in lung cancer tissues. A significant difference was observed between the two groups (p<0.05). In squamous cancer and adenocarcinoma, the positive rates of COX-2 expression were 40.0% and 75.0% respectively. The difference between them was significant (p<0.05). In well, moderately and poorly-differentiated squamous cancer groups, the positive rates were 18.8%, 35.7% and 66.7% respectively. In well and poorly-differentiated adenocarcinoma groups, the positive rates were 58.8%, 87.0% respectively .In the group with and without lymph node metastasis, the rates were 37.1% and 70.0% respectively. The difference between them was significant (p<0.05).2, The positive staining of P53 mainly located in the nucleus of lung carcinoma cells with the positive rate of 58.8%. There were no expression of P53 in benign lung lesions. In the groups with and without lymph node metastasis, the positive rate were37.1% and 74.0% respectively, the difference between them was significant (p<0.05). In well, moderately and poorly-differentiated squmaous cancer groups, the positive rate were 56.3%, 64.3% and 53.3% respectively. In well and poorly-differentiated adenocarcinoma groups, the positive rates were 58. 8% , 60.9% respectively.and no significant difference was observed between them (p>0.05). |
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