| Background and ObjectiveThe occurrence of lung carcinoma increased notably and it does severely harm to humankind recently. The carcinogenesis of lung tissue is a progression with many steps and phases and its mechanism is rather complex. Researches have shown that activation of oncogenes and inactivation of tumor suppressor genes are involved in it. PTEN, a recently identified tumor suppress gene (TSG), has complex functions including participating in the embryonic growth, apoptosis and Gl phase cellblock, inhibiting the migration and adherence. The newborn vessels can supply nutrition to tumors, which is the prerequisite to tumor metastasis and can stimulate the growth of tumor. Angiogenesis is one of crucial steps to the carcinogenesis and tumor progression, and PI3K/AKT signal pathway is essential to angiogenesis. PTEN inhibits the PI3K/AKT pathway so it may inhibit the angiogenesis. But until now the relations between PTEN and angiogenesis in lung cancer have not been reported. The wide-type p53 gene plays an important role in maintainingnormal cell growth and inhibiting the malignant proliferation. The wide type p53 gene promotes vessel formations by up-regulating vascular endothelial growth factor (VEGF). Only mutant type p53 gene can be detected by immunohistochemical method. The expressions of PTEN, p53, and micro vessel density (MVD) were analyzed by immunohistochemical method to explore the mechanism of lung carcinogenesis.Materials and Methods75 surgically resected non-small cell lung carcinoma tissues were collected. Among them male 55, female 20; Of them, 40 cases were squamous cancer, 35 cases were adenocarcinoma; In addition, of them, 25 cases were well-differentiated adenocarcinoma, 10 cases were poorly-differentiated adenocarcinoma; 14 cases were well-differentiated squamous cancer, 13 cases were moderately-differentiated squamous cancer, 13 cases were poorly-differentiated squamous cancer; And there were lymph nodes metastasis in 45 cases, no lymph nodes metastasis in 30 cases; 20 cases inflammatory lung lesions were collected as control groups. All the tissues were fixed in 10% neutral formalin and embedded in paraffin. SP immunohistochemical method was performed to detect the expressions of PTEN, p53 and CD34. The data were analyzed by software SPSS 10.0, Chi-square Test and exact probability method were used to compare the difference of PTEN and p53 expressions between groups, f-test was used to compare the difference of MVD between groups. =0.05 was considered as the level of tests.Results1 . The positive staining of PTEN mainly located in cytoplasm. The rate of PTEN expression was 95.0% in inflammatory lung lesions. The positive expression was only 48.0% in lung cancer tissues. A significant difference was observed between the two groups (P<0.05). In squamous cancer and adenocarcinoma, the positive rates of PTEN expression were 55.0% and 40.0% respectively. There was no significant differencebetween them (P>0.05). In well, moderately and poorly-differentiated squamous cancer groups, the positive rates were 92.9%, 46.2% and 23.1% respectively. In well and poorly-differentiated adenocarcinoma groups, the positive rates were 52.0%, 10.0% respectively. In the group with and without lymph nodes metastasis, the rates were 35.6% and 66.7% respectively. The difference between them was significant (PO.05).2. The positive staining of p53 mainly located in the nucleus of lung carcinoma cells with the positive rate of 53.3%. There were no expressions of p53 in normal lung tissues and a significant difference was found between them (.PO.05). In the groups with and without lymph nodes metastasis, the positive rate were 68.9% and 30.0% respectively, the difference between them was significant (P<0.05). In well, moderately and poorly-differentiated squamous cancer groups, the positive rates were 35.7%, 76.9% and 46.2% respectively. In well and poorly-differentiated adenocarcinoma groups, the positive rates were 56.0% , 50.0% respectively, and no si... |
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