The Expression Of MVD/S100 Protein And VEGF In Colorectal Neoplasm And Their Clinical Significances

Objective To investigate the expression of microvessel density(MVD) and vascular endothelial growth factor (VEGF) and s100 protein in colorectal neoplasm and their clinical significances.Methods From Jan. 1994 to Jan.1995,we gathered 59 colorectal cancer paraffinimbeded tissue samples which had been diagnosised accurately from gerneral surgery of Qilu Hospital of Shandong University. The expression of MVD/ s100 protein and VEGF in 59 patients with colorectal cancer and 16 patients with adenomas and 12 normal patients were investigated using immunohistochemical staining.Results The positive rates of VEGF/ s100 protein and MVD in colorectal cancer were significantly higher than those adenomas (P<0. 05). It was significantly different from Dukes stage, it was significantly higher in Dukes C, D stage than in A stage, A+B stage than C+D stage (P <0.05).MVD and s100 protein were correlated with differentiation, but VEGF were not.It was significantly different,the positive rates of MVD/S 100 protein and VEGF in colorectal cancer with lymph nodeor liver metastasis were higher than without (P<0.05>. It was remarkably different between the cases with recurrence and without (P<0.05>. the expressions of VEGF were related to Duke's stage. MVD was related to Duke's stage carcinoma position, pathology type, differentiation and infiltrate depth.Conclusion1 .The results suggested that VEGF accelerated neovascularization strongly and played an active role on the occurrence and development of colorectal carcinoma. Lymph node metastasis was related clearly to the VEGF express in colorectal carcinoma. The detections of VEGF and MVD may help predict clinical prognosis.2. The results also showed that colorectal lymph node metastase obtained more strongly malignant invasion because of the high expression of VEGF.3. S100 protein may serve as a new useful marker in judging the degree of malignancy and invasion of colorectal neoplasm .