The Preventive And Therapeutic Effects Of Hyperoxygen Solution On Hypoxia During One-lung Ventilation In Dogs

[Objective ]To investigate the preventive and therapeutic effects of hyperoxygen solution on hypoxia during onerlung ventilation (OLV) in dogs.[Methods ]Experimental dogs were anesthetized with 2% pentobarbital and inserted double-lumen endotracheal tube (Mallinckrodt), and maintained respiration with respirator. Then arterial and 4Fswan-Ganz venous catheters were inserted via right femoral artery and right internal jugular vein to monitor MAP, SvO2, MPAP, PCWP, PvO2, SvO2, ECG and blood gas analysis.Experiment 1: The preventive effects of hyperoxygen solution on hypoxia during one-lung ventilation in dogs15 dogs were randomly divided into three groups. Control group (n=5): intravenous injection of 200ml 0.9% saline. Hyperoxygen solution group (n=5): intravenous injection of 200ml hyperoxygensolution. Jingshuyang group (n=5): intravenous injection of 200ml Jingshuyang. All the animals were performed OLV immediately after the transfusion. HR, MAP, CO, MPAP, PCWP and CVP were recorded before OLV, at 15 min and 30 min after OLV. Venous samples were collected to determine blood serum LA, MDA and SOD concentration before OLV, 15 min and 30 min after OLV.Experiment 2: The therapeutic effects of hyperoxygen solution on hypoxia during one-lung ventilation in dogs15 dogs were randomly divided into three groups. Control group (n=5): intravenous injection of 200ml 0.9% saline. Hyperoxygen solution group (n=5): intravenous injection of 200ml hyperoxygen solution. Jingshuyang group (n=5): intravenous injection of 200ml Jingshuyang. Animals were infused above liquids immediately at 30min after OLV. HR, MAP, CO, MPAP, PCWP and CVP were recorded before OLV, at 30 min after OLV and 20 min after infusing above liquids. At the same time, arterious, venous and arterio-venous samples were collected to determine pH, PaO2, PaCOa, PvOz, SvO2, SaOa respectively. Venous samples were also collected to determine blood serum LA, MDA and SOD concentration before OLV, 30 min after OLV and 20 min after infusion.[Results ]Experiment 1:(1) Influence of Hyperoxygen solution on hemodynamics. PCWP, PAP increased significantly at 15 min and 30 min after OLVin saline infusion group (P<0.05), but remained stable after infusing hyperoxygen liquid and Jingshuyang.(2) Influence of Hyperoxygen solution on blood gas.Compared with the data before OLV, SaO2, PaO2, PvO2, SvOa weresignificantly decreased in group of 0.9% saline, while PaCO2 significantly increased (P<0.05) at 15 min following OLV and pH remained unchanged, but significantly decreased at 30 min after OLV. In groups of hyperoxygen liquid and Jingshuyang, all of above parameters remained stable at 15 min after OLV, but in Jingshuyang group, SaO2, PaO2 were significantly reduced and PaCO2 was significantly increased at 30 min after OLV (P<0.05).(3) Influence of Hyperoxygen solution on plasma biochemical indexes.Compared with the data before OLV, blood serum LA, MDA increased and SOD decreased significantly at 15min and 30min after OLV in saline group (P<0.05). LA, MDA, SOD value remained unchanged at 15min after OLV in hyperoxygen liquid and Jingshuyang groups, but LA value increased at 30 min after OLV in Jingshuyang group (P<0.05). There were significanlly difference between hyperoxygen liquid and Jingshuyang group in LA values at OLV 30min.Experiment 2:(1) Influence of Hyperoxygen solution on hemodynamics during OLV.PC\VT\ PAP increased significantly at OLV 30 min and 10 min after infusion, and continued increasing in saline group (P<0.05), but returned to the levels before OLV after infusing hyperoxygen liquid and Jingshuyang. There were also significantly difference in PCWP, PAP values between hyperoxygen liquid and Jingshuyang group at 20 min after infusion (P<0.05), other parameters remained stable.(2) Influence of Hyperoxygen solution on blood gas during OLV. Compared with the data before OLV, SaO2, PaO2, PvO2, SvO2 weresignificantly decreased in three groups, while PaCO2 significantly increased at 30 min following OL...