Study Partial Liquid Ventilation For Rats With Endotoxin-induced Lung Injury

Objective: To investigate the partial liquid ventilation and its effect on the ultrastructure of PS system in rats with endotoxinnduced lung injury. Methods: Twentyour Sprague Dawley rats were aneasthesized and ventilated, and randomly assigned to acute lung injury (ALl) group and partial liquid ventilation (PLy) group after endotoxin was injected through jugular vein. All the animals received conventional mechanical ventilation,but in PLV group the administration of perfluorocarbon lOml/kg was given intratracheally at 30mm following endotOxin injection. Blood were sampled to measure arterial blood gas , and to count white blood cells and hemodynamic(MAP and HR) monitored at 0. 5h, lh, 2h, 4h and 6h following endotxoin injection. And lung tissues were taken after the experiment ot assess the pathology and to observe the electromieroscopic histochemistry. Results: There were not marked differences for MAP and HR between the two groups. PLY could significantly improve the Pa02 and arterial pH deterioration caused by endotoxin.The lung 3 inf1amatory response in PLV group seemed 1ess significantthan that in ALI group. The pulmonary surfactant layer wasdiscontinuous, 1anellar bodies and plasmosomes in type I1pneumonocytes vacuo1ated, and a few of them even necrotizedand disrupted into the alveolar space in ALI group. The aboveiIllPairment of PS system was less significantly in PLV gToup.Conc1usion: PLV cou1d markedly improve the gas exchange andreduce the lung inf1anunatory response, and 1essen theiIIlPairment of PS system in rats with endotoxin--induced ALI.