| Objective To provide corneal epithelial transplant forcorneal surface reconstruction .Method The amniotic membrane preserved in glycerinwas used as a carrier for growing limbal corneal epithelial cells in vitro.Results Histological and ultrastructural study of the cultured corneal epithelium showed that the cultured corneal epithelium was quite simil-ar in histological structure and ultrastructural to those in vivo.Conclusion The corneal epithelium cultured on theamniotic membrane may prove to be potential grafts for corneal epithelium transplantation. |