The Effects Of Polyethylene Glycol400on The Healing Of Corneal Epithelium

Objective To investigate the effects of polyethylene glycol400on the biologicalcharacteristics of corneal epithelial cells (CECs) and molecular mechanisms thereof.Methods Polyethylene glycol400was diluted to0.1%with DMEM/F12medium.HCECs were cultured by these medium. DMEM/F12medium without polyethylene glycol400was control group. The effects were tested by four ways. Scarification was used to testthe ability of cell migration; the MTT assay and flow cytometer were respectively used totest the effect on growth curve and cell cycle; RT-PCR was used to measure the expressionof basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in Humancorneal epithelial cells (HCECs).Result0.1%Polyethylene glycol400has no discernable effects in the morphologyof CECs. Cell migration of CECs was improved by0.1%Polyethylene glycol400. CECscultured in the dilution of polyethylene glycol400solutions and cell cyle measurementsshow that0.1%polyethylene glycol400promotes in vitro HCEC growth(P=0.0072;P=0.0034) and up-regulates the expression of bFGF and EGF in cultured CECs(P<0.05;P<0.01).Conclusion Polyethylene glycol400does not affect cell morphology and it promotesCEC proliferation via reducing cell cycle and increasing bFGF and EGF expression. Objective To investigate the effects of0.4%polyethylene glycol eye drop on thehealing of mechanical wound corneal epithelium of mice. Methods30KM mice were selected for this test. Experiment contained three groupsincluded0.4%polyethylene glycol eye drop group, NS group and control group. And eachgroup had10KM mice.we built the structure of defection of corneal epithelium by curette.We observed corneal healing from shooting under slit lamp after6h,12h,24h,48h and72h.Result The valid rate of all groups were zero in6h. In12h this rate of0.4%polyethylene glycol eye drop group was75%and the other groups were still zero, the rateof0.4%polyethylene glycol eye drop group became100%and the other groups were both16.7%, both differeces of these times had statistical meaning(P=0.001; P=0.001). And in48and72h all of groups were100%.Conclusion0.4%polyethylene glycol eye drop could speed repair of cornealepithelium after injury. Objective To investigate the effects of0.4%polyethylene glycol eye drop on thehealing of burning of corneal epithelium with20%alcohol.Methods30KM mice were selected for this test. Experiment contained three groupsincluded0.4%polyethylene glycol eye drop group, NS group and control group. And eachgroup had10KM mice.We built the structure of burning of corneal epithelium withfilter-paper contain20%alcohol. We executed three mice in each group after1d,3d and7d.We picked their eyes with HE stain and immunohistochemistry in JAM, bFGF andEGF.Result The expression of bFGF and EGF in the group of0.4%polyethylene glycoleye drop was more higher than the group of NS and control group after wound in one day.The difference had statistical meaning(F=29.853, P=0.001; F=123.965, P=0.000). Theexpression of JAM had no difference among three groups(F=0.735, P=0.589). After woundin three days the expression of bFGF, EGF and JAM all had no difference among threegroups(F=3.694, P=0.062; F=1.639, P=0.256; F=1.307,P=0.332) as same as in seven days(F=0.644, P=0.608; F=0.413, P=0.679; F=0.207, P=0.929).Conclusion It could up-regulate the expression of bFGF and EGF after20%alcoholburning. But it had no effects on the expression on JAM.