Studies On Effects Of Yqtld On Delayed Neuronal Death Of Hippocampus Of Transient Global Forebrain Ischemia Of Rat

Objective: To investigate effect of Yiqi Tongluo Decoction (YQTLD), a traditional Chinese medicinal prescription, on delayed neuronal death of hippocampus of transient global forebrain ischemia in rat. Methods: Transient forebrain ischemia was produced using published modifications of the four-vessel occlusion (4-VO) method and it triggered the delayed death of CAl neurons in the hippocampus. YQTLD was given orally to rats. Then, we studied the effect of YQTLD on cell survival and DNA fragmentation in the hippocampus in a rat model of transient global ischemia and on expression of caspase-3P20 in CAl neurons, indicative of apoptosis. Results: (1)oral administration of YQTLD has effect on cell survival. However, YQTLD significantly intensified the number of survival neurons. (2) YQTLD markedly attenuated the number of TUNEL positive neurons. (3) the expression of caspase-3P20 decreased in CAl nuerons after transient forebrain ischemia of oral administration rats of YQTLD. Conclusion: The present results suggested that YQTLD given orally prevented the necorsis and apoptosis and prevented caspase-3P20, the production of catalytically active caspase-3, which might account for the enhancing effect of YQTLD on cell survival by preventing caspae-3 way.