A Study On Experimental Diabetes Animal Models In Tree Shrew Induced By Streptozotocin

Objective: To study the standard of fasting blood glucose (FBG) and the proper dosage of streptozotocin (STZ) in the diabetic model in tree shrew induced by STZ. Methods: 45 tree shrews were divided at random into normal control group intraperitoneally (i.p.) injected with acid normal saline and diabetes groups: D80, D100, D120, D150 i.p. injected with STZ at the dose of 80, 100, 120, 150mg/kg body weight, respectively. The observed time was 10 weeks. Their FBG were observed systematically before and after they were i.p. injected with STZ. 4 and 10 weeks after ip injected with STZ, some tree shrews were killed, and the pathological changes of their pancreas were observed with light microscope and electron microscope. Results: The tree shrews whose FBG≥11.1mmol/L developed classical diabetic symptoms and showed hyperglycemia lasting at least for 4 weeks without recovery, and the pathological studies of their pancreas showed reduction of islets, degeneration of islet cells, interstitial fibrosis and hypoplasia of islet cell. The results also showed that the incidence was D80(D100D120 >D150, and D150 was 62.5%; the rate of death 3 was the highest in Dl 50, that reached 11.1%. Conclusion: the standard of diabetic model in tree shrew is FBG≥11.1mmol/L, and the proper dosage of STZ is 150mg/kg body weight.