Eliminate Labor Eyes Granules Experimental Tree Shrew Liver Model Workers Pupillary Sphincter

Object: To investigate the effect of xiaolaoyanshukeli(消劳眼舒颗粒) on the sphinctre muscle of Tree Shrew and discuss the mechanism.Methods:Tree Shrew was made staring continuously.They were divided into 6 groups randomly: nomal control group, model group (negative control group),positive control group , three xiaolaoyanshukeli groups , including high dose, middle dose and low dose. Iris of Tree Shrew was separated to quantitate the MAChR by radioimmunossay and the pathologic histology changes of sphinctre muscle were oberved by Mias-2000 image analysis system.Results: (1) Compared with the nomal control group, the Kd of MAChR in the sphinctre muscle of Tree Shrew in model group reduced (P <0.05).Compared with the model group, the Kd of MAChR in all therapy groups significantly improved (P<0.01,0.05). The RT of MAChR in all groups was not statistic value .(2) Compared with the model group ,the average optical density in HE staining of the iris sphinctre muscle in all traditional Chinese medicine therapy groups significantly decreased (P<0.01,0.05) .The average optical density and the average grey level in Masson staining of all groups were not statistic value; Compared with the model group , the average grey level in a-SMA staining of the iris sphinctre muscle in all therapy groups significantly decreased(P<0.01,0.05).Conclusion: The persistent erethism of parasympatheti controlling iris sphincter muscle may be one of important reasons of GanLao (肝劳) .Xiaolaoyanshukeli could adjust the excitability of parasympathetic nerve and relax contracting muscle.