Establish The Regeneration Systems Of Two Ophiopogon Japonicus And The Preliminarily Study On Radiation Induction Of Ophiopogon Japonicus 'Nanus'

In this study, Ophiopogon japonicus'Nanus'and Ophiopogon japonicus (L.f.)Ker Gawl were used as study materials. And they have been established for the regeneration systems through selecting optimal culture medium for induction and differentiation of calluses. Therefor it brings some theoretical basis for enlarging extended planting area. Then different doses of 60Co-γradial were used to irradiate the callus and plant of Ophiopogon japonicus'Nanus'. Through calculating the LD50 and observing the plant's external morpha, good methods have been explored for radiation induction of 60Co-γradial on Ophiopogon japonicus'Nanus', and it will provide important radiation induction data in theory. The results are as follows:1. The results of establishing regeneration system for Ophiopogon japonicus'Nanus'are showed that the best disinfection way for explants is as follow: 20% sodium hypochlorite for 10min, 70% alcohol for 0s, 0.1% HgCl2 for 8min, and basal leaves are the best explants, then the best culture medium for induction of calluses is composed of MS+0.2mg/L6-BA+1.0mg/LNAA+30g/LSucrose+6g/LAgar, also it has been found that callus induction rate should be positively correlated with treatment temperature and negatively correlated with treatment number of days while the appropriate culture mediums for inductuin and differentiation of buds are respectively composed of MS+3mg/L6-BA+0.1mg/LNAA+30g/LSucrose+6g/LAgar and MS+ 3mg/L6-BA+0.05mg/LNAA+30g/LSucrose+6g/LAgar, and they can reach more than 70% rate of differenciation, and the highest multiplication coefficient medium is comprised MS+3mg/L6-BA+0.05mg/LNAA+30g/LSucrose+6g/LAgar, also the best rooting medium is composed of 1/2MS +0.05mg/L NAA + 30g/L Sucrose+6g/LAgar and 1/2MS+0.02mg/LNAA+30g/LSucrose+6g/LAgar.2. The results of establishing regeneration system for Ophiopogon japonicus (L.f.) Ker Gawl are showe that the best disnfection way for explants is as follow: 20% sodium hypochlorite for 10min, 70% alcohol for 20s, 0.1% HgCl2 for 8min, then basal leaves are the best explants, and the best culture medium for induction of calluses is composed of MS+0.2mg/L6-BA+0.5mg/LNAA+30g/LSucrose+6g/LAgar, and then the best culture medium for differentiation of buds are compoed of MS+0.5mg/L6 -BA+0.1mg/LNAA+30g/LSucrose+6g/LAgar.3. The influence of radiation induction on embryo induction and plant of Ophiopogon japonicus'Nanus'also suggests that the more does of 60Co-γradial, the less differentiation of bud and the LD50 is 8Gy. And for plant, and these is different degree damage from 15Gy to 30Gy and from 5Gy to 15Gy, also it brings some change with plant morpha, leaves height, leaf width, number of leaves and so on.