Determination Of Inorganic Elements And Analysis Of RAPD And SRAP On Different Populations Of Ophiopogon Japonicus

Ophiopogon japonicus(L.f)Ker-Gawl commonly called dwarf lily turf, is a perennial herb that belongs to Ophiopogon, the large Liliaceae family. It is recorded in previous edition of Chinese Pharmacopoeia. Dwarf lily turf is commonly used its root as medicine. It is cured for nourish yin, moisten lung, relieve restlessness thrombosis and so forth. Dwarf lily turf, which is mainly distributed in Sichuan, Zhejiang and other places of China. This paper detected the contents of inorganic elements on different regions of25O. japonicus wild materials from Sichuan province and Chongqing; studying the geneitic diversity using RAPD and SRAP-two kinds of molecular markers. In order to provide. theory and basic datum for better resources and cultivate breed.The specific results of these studies are as follow:1. Detecting the contents of25O. japonicus materials from different strains. Showed that the contens of inorganic elements is different from the different strains. K、Ca、Na、 Fe、Mg、Zn the six elements have higher average contents. The content of inorganic elements in spreading type is higher than erect type in the local area. Spreading type is better choose as cultivated plants in the production.2. Nine RAPD random primers, selected from twenty primers, are used to amplify24O. japonicus materials on different populations and109DNA bands are amplified, of which polymorphic bands are105and the percentage of polymorphic loci is96.3%. Every primer amplifies approximately7to15bands with an average of11.7bands for each primer. The genetic similarity ranges from0.0361-0.9389with an average of0.4920based on RAPD. Used RAPD molecular markers to analyze genetic clustering and showed that24O. japonicus materials from different strains are divided into two parts. Cultivar species NO.2and NO.3from Santai are clustered together and has closer relationship. Their genetic distance are far away from other wild resources.3.33SRAP primers pairs selected from169primers pairs are used to amplify the24O. japonicus materials and325DNA bands are amplified, of which polymorphic bands are317and the percentage of polymorphic loci is97.5%. Every primer pair amplifies approximately7to14bands with an average of9.6bands for each primer. The genetic similarity ranges from0.4193-0.9503with an average of0.6567based on SRAP. Used SRAP molecular markers to analyze genetic clustering and showed that24O. japonicus materials from different strains are divided into two parts. There are closer relationship between NO.2and NO.4, which can be clustered together.4. The results demonstrate that there is genetic differentiation on a certain extent among the population of O. japonicus. Cluster is carried out based on genetic diagram and the correlation coefficient within the populations, it is showed that clustering results are related to morphological characteristics, but not to geographic regions.