Preliminary Study On Diet - Induced Metabolic Syndrome Rat Model

Objective：The metabolic syndrome animal model for the prevention of disease and drugscreening is of great significance, which is becoming a research hotspot. This studychooses diet candidated to set up a metabolic syndrome animal model, by comparingthe different formula and different concentration of sucrose solution on experimental,watching a variety of indicators of SD rats and Wistar rats. The purpose is to find asimple, stable, high rate model of metabolic syndrome in rats.Method：1.30SD rats were randomly divided into4groups according to weight: controlgroup7, feeding ordinary feed; Model group (1)7, feeding high fat and sugar highsalt feed; Model group (2)8, a feeding high purine feed; Model group (3)8, feedingno.2high purine feed. Synchronous testing water content, food intake and bodyweight changes. In building14D and28D, eye plexus take blood sinus, separation,serum detection of serum glucose (Glu), triglyceride (TG), uric acid (UA), and highdensity lipoprotein (D-HDL-C) four indicators.2. According to the previous experimental results,30SD rats were randomlydivided into4groups according to weight: control group (n=10), model group (1)(n=10) and model group (2)(n=10). Control group fed chow, building model group (1)feeding1feed, model group (2) feeding the no.2building feed. Atotal of16weeks.Observation rat activity and feeding conditions. Since4weeks, determinating zhedaily amount of drinking water and food intake about rats. Measuring weight onceevery two weeks. Every two weeks, eye plexus take blood sinus, separation, serumdetection of serum glucose (Glu), triglyceride (TG), uric acid (UA). In12,14,16weeks rats on metabolic cages, blank group10, selected6high blood sugar of twomodle groups, determination of24h urine trace albumin.Compared the differencesand similarities, and made a preliminary evaluation.3. According to the previous experimental results,60SD rats were randomlydivided into4groups according to weight, each group of12. Control group drinkingplain water, sucrose1group,2group and3group drinking respectively30%,40%and30%concentration of sucrose solution, four groups were fed chow; High fat and sugar group drinking plain water, feed feeding high fat and sugar. Atotal of18weeks.To observe the general situation of rats and weight changes in2,3,5,8,10,16,18weeks testing rats serum glucose (Glu), triglyceride (TG) and uric acid (UA).16and18weeks added measure high-density lipoprotein cholesterol (HDL-C) and24h urinetrace albumin.18weeks executed, weighing the kidneys and liver wet weight.4. According to the previous experimental results,36Wistar rats were randomlydivided into4groups according to weight, each group of12.Control group feedingand drinking plain water, two groups of model group:1and no.2groups weredrinking30%and40%concentration of sucrose solution. Three groups were fed chow.Atotal of8weeks. The general condition and weight change, observed in the rat in2、4、6week, detection of rat serum glucose (Glu), triglyceride (TG), uric acid (UA) andhigh density lipoprotein cholesterol (HDL-C).5. Comparing model groups of SD rats and Wistar rats: the1group and2group.in experiment2w and8w, comparing glucose (Glu), triglyceride (TG), uric acid (UA)index, the purpose is to select a more ideal model rats.Result：1. Comparing three formula made mould feed to SD rat model of metabolicdisorders. Modeling,14, and28days compared with the control group, model grouprats①uric acid, triglycerides and blood glucose levels were significantly increased(P <0.05, P <0.01); model group②uric acid in rats, triglyceride levels wereincreased trend, but no significant difference; model group③uric acid in rats,triglycerides, blood glucose levels were not significantly different.2. Two kinds of fat and sugar food have feed SD rats for6weeks, all can causeSD rats with different degree of metabolic disorders(P<0.05,P<0.01);Among them,the model group (1) in serum Glu and TG change more obvious, model group (2) thechanges in serum UAand24h urine trace albumin on performance is more obvious.3. Compared with the blank control group, different concentrations of sucrosesolution can cause Wistar rats with different degree of blood sugar、fat and uric acidmetabolic disorder (P<0.05, P<0.01),urine trace albumin increased(P<0.05,P<0.01),kidney weight ratio decreased (P<0.05,P<0.01), liver index to rise(P<0.05,P<0.01).The HDL-C of high fat and sugar group was obviously higher, other three model groups were significantly lower (P<0.05,P<0.01). Number one sugar groupweight increases rapidly to obesity.4. Compared with the blank control group, different concentrations of sucrosesolution can cause Wistar rats with different degree of blood sugar、fat and uric acidmetabolic disorder. Number one sugar group weight increases rapidly to obesity.5. The same concentration of sucrose feeding different varieties of rats, serumtriglyceride (TG) in Wistar rats at2,8weeks is significantly higher than SD rats (P <0.05, P <0.01), serum glucose (Glu), uric acid (UA) has no obvious change.Conclusion：1. Comparing the rise and fall of the four serum indices about the three kinds offormula feed of SD rats. Feeding high fat and sugar feed group rats most conforms tothe metabolic syndrome diagnostic criteria, and close to the human diet compositionand way of building effect is ideal.2. Long-term intake of high fat and sugar food can lead to blood sugar and fatmetabolism disorder, cause relevant index increased, and high uric acid hematicdisease, cause elevated urine trace albumin, kidney weight ratio decreased, liver indexto rise. Two kinds of feeding high fat and sugar can cause comprehensive metabolicdisorders in the short term, moreover, the researchers can choose according to theneeds of the experiment.3. Using the concentration of30%sucrose solution feed SD rats can causeobesity and different degree of blood sugar、fat and uric acid metabolic disorder in ashort time. This model is similar to human clinical characteristics, conform to therequirements of the MS definition, suitable for as a treatment and intervention of drugscreening model.4. Using the concentration of30%sucrose solution feed Wistar rats can causemetabolism disorder in a short time. Among them,30%sucrose solution can causeobesity rats, the modeling time is the shorter than the SD rats, conforms to thecharacteristics of MS.5. Sucrose feeding different varieties construction of metabolic syndrome in ratsmodel of Wistar rat modeling time is short, compared with SD rats serum TG level isgrowing rapidly. 6. Using the concentration of30%sucrose solution feeding Wistar rats six weeks,can build give a kind of simple and stable animal model of metabolic syndrome.