Influence Of High-Fat And High-Protein Diet On Glucose Metabolism Of Rats At Development Stage

PrefaceWith the development of economy and increasing of people's life,changes of dietary structure and lifestyle,obesity in children and adolescents is becoming a major epidemic.Obesity is associated with problems of adult cardiovascular disease and increase its younger age,as well as high blood pressure,lipid disorders,diabetes and other commonly found in adults of cardiovascular disease risk factors to extend in children and adolescents.In recent years a growing research confirmed that the nutritional status of early life have lifelong effects on the human body metabolism of adult obesity and other chronic diseases of great significance.Many scholars from animal experiments and clinical researchs confirmed the fetal period and the period of infant have important influence on the future growth and development of certain adult diseases.Critical period of growth and development,but also nutrition program of the sensitive period that nutrients for the body to stimulate the potential of individual growth and development,and it have long-term impact on body until adulthood and become the theoretical basis on prevention of adult diseases in childhood.This paper by giving young age of 5-6w rats high-fat,high-protein diet to study the effects on growth and development and insulin resistance,in order to provide a theoretical basis for the early prevention of adult diseases.Materials and Methods1.Animalforty-eight male rats,weighing 80-100g(purchased in China Medical University Animal Center),clean-class animal.Male Wistar rats were randomly divided into control group,high-fat group and high-protein group after born 5-6 weeks according to body weight,maintained respectively a standard,a high-fat and a high-protein diet for 12 weeks.Weight,food intake and water intake recorded once a week.Rat 2/cage farming,under temperature(20±2)℃,humidity 55%- 65%,the daily maintenance of 12h light/dark cycle,free food,drinking water.2.Collecting samplesCollecting vein blood serum of three group after 12h no food in 6 weeks and 12 weeks respectively.Serum was obtained by low-speed centrifugation(3000r;20 minutes) and were stored at -70℃refrigerator.The liver,kidney,brain was removed and washed with cold saline,quickly blotted and weighed.At the same time,removed perirenal and epididymal fat pad and weighed them,organ coefficient was calculated accordingly.3.Assay methodsThe levels of glucose were measured by neighbor toluidine,insulin were measured by radioimmunoassay,the level of serum cholesterol and triglyceride were measured by enzymatic method.4.The judgement standard of IRWe used HOMA-IR-FINS×FBS/22.5 to reflect insulin resisitant.The more numerical value,the more insensitive to insulin.5.Statistical methodThe significance of differences among groups were analyzed statistically by ANOVA,results are expressed as(?)±S.Comparison between two groups using LSI) test.0.05 represent statistics significance.Results1.The changes of body weight in different feed groupAll rats from start to the 12th weekend,there was no statistical difference in body weight(P>0.05).2.The changes of food consumption and calorie intake in different feed groupDifferent feed group of rats for 6 weeks,compared with control group,daily food consumption in high-protein group did not have statistics significance(P<0.05);There was lower daily food consumption in high-fat group,the difference have statistics significance(P<0.01);Different feed group to compare the food titre,the difference did not have statistics significance(P>0.05).Three groups of rats for 12 weeks,compared with control group,daily food consumption in high-protein group did not have statistics significance(P>0.05);There was lower daily food consumption in high-fat group,the difference have statistics significance(P<0.01);Compared with control group,there was higher food titre in high-fat group,the difference have statistics significance(P<0.05).3.The changes of organ coefficient in different feed groupDifferent feed group to compare the liver coefficient,the brain coefficient,the kidney coefficient,the difference did not have statistics significance(P>0.05);Different feed group of rats for 6 weeks,compared with control group,there were higher perirenal fat weight,epididymal fat pad in the high-fat group and high-protein group(P<0.01 or P<0.05).Different feed group of rats for 12 weeks,compared with control group,there were higher perirenal fat weight,epididymal fat pad in the high-fat group(P<0.05).4.The changes of blood lipid,blood glucose,insulin concentration and IRI in different feed groupDifferent feed group of rats for 6 weeks,different feed group to compare the level of serum cholesterol and triglyceride,the difference did not have statistics significance(P>0.05);Compared with control group,there was higher blood glucose in the high-fat group and high-protein group(P<0.01),IRI of high-fat group and high-protein group(P<0.05 or P<0.01) was higher than that in control group.12 weeks,compared with control group,there were higher level of serum cholesterol and triglyceride in high-fat group,the difference have statistics significance(P<0.05);Blood glucose in the high-fat group and the high-protein group was higher than that in the control group,but the difference did not have statistics significance(P>0.05);Insulin concentration and IRI of high-fat group and high-protein group were higher than that in control group(P<0.05 or P<0.01).Conclusions1.Infant rats fed with high protein diet for a long time had no impact on body weight and important organs,but increased body fat content,caused insulin resistance.2.Infant rats fed with high fat diet for a long time increased body fat content and the level of serum cholesterol and triglyceride,led to insulin resistance.