Study On Tissue Culture In Phalaenopsis

In this experiments, we choosed to use the Phalaenopsis in vitro culture. These aspects of first step researching were proceed: inducenment flower stalks with dormancy buds prouting, inducing protocorm-like-body(PLB) from leaves, propagating PLB, rooting of PLB .The results are shown as below:1. Inducing dormancy buds from flower stalkWe used the flower stalks with dormancy buds which selected in summer, autumn and spring to culture. Comparing their contamination rate, sprouting rate and growth condition of buds, proved that it is best to use the flower stalks which selected in autumn to culture. The effect of sterilization is the best and the induce rate of dormancy buds is the higliest. MS medium supplemented with 5.0 mg.L-1 BA were fit to induce sprouting.2. Inducing PLB from leaves5.0mg.L-1 BA is fit to induce PLB from tube-culture Phalaenopsis leaves. Appending 15% (v/v) coconut water (CW) can enhance the induce rate and advance the growth of the PLB. In MS+BA 5.0+CW 15% medium adapt to induce PLB from leaves, inducing rate is 38%.3. Multiplication of PLBBA and juice can prove the multiplication of PLB. Poly ( N-Viny 1-2-Pyrrolidinone) (PVP) can prevent the hydroxybenzene pollution in medium and prove the multiplication rate. MS+BA 5.0+CW 15% medium is suitable for PLB mutiplication.4. Rooting1/2 MS medium supplemented with 0.1-0.5 mg.L-1 NAA or MET can induce rooting of PLB. When rooting induced by paclobutrazol, seedlings were short and strong, leaves were greener than others. It promotes the survival rate of transplantation. Appending 0.3 g.L-1 active carbon (AC) can promote the average amount of roots and average root length.1/2 MS+BA 2.0+NAA 0.1+AC or 1/2 MS+BA 2.0+MET 0.3 medium are suitable for induce rooting.5. TransplantationMoisture and permeability affect the survival rate of tube-plantlet in transplantation. High degree of moisture and low permeability would lead to the death of plantlet.