Establishment Of Tissue Culture Regeneration System And Technique For Commercial Production Of Phalaenopsis Hybrimycin

This paper is concerning on the different factors associate with the tissue culture of Phalaenopsis hybrimycin. The types of medium and combination levels of different hormone are experimented during the different culturing stages of Phalaenopsis hybrimycin for different purposes, such as the induction of callus from different explants, induction and multiplication of protocorm, In vitro rooting of shoots and so on.Ecological factors(temperature, humidity and light intensity ) , nutrition controlling and disease and pest control are also measured during the procession of commercial production . The results show that:1. Axillary bud and intersegment of flower—stem are the better explants for tissue culture for their easy treatment and little damage during sterilization. Medium (Hyponex: N-P-K: 6.5-6-19) 2.5g/L supplemented with BA 3.0mg/L is suitable for induction of axillary bud. Medium supplemented with BA3.0mg/L+NAA0.2mg/L has the better effect to the differentiation of indefinite bud.2. Medium Hyponex with low concentration of inorganic salt ,supplemented with 6—BA2.0mg/L+NAA0.5mg/L is suitable for the multiplication of protocorm. Multiple index can reach more than 5 times within 2 months.3. The medium (N-P-K: 20-20-20)2.7g/L supplemented with 6-BA 0.1mgL + NAA 0.5mg/L is the better medium for rooting ,with high rooting rate, more roots.4. Liquid medium without agar is used for culture of in vitro plant before field setting, for this kind of medium has many advantages such as strong growth potential for plant, more roots, easy clean before transplantation, fastrestoration of plant after transplantation, and high survival rate.5. Aquatic weed with Good hydroscopic properties, water retention property and acidic pH make it the best matrix for cultivation of Phalaenopsis hybrimycin.6. In order to obtain the good quality potted plants, many measures are necessary, such as controlling and balancing temperature, humidity and light in greenhouse, supplementation of nutrition corresponded and disease and pest controlled.