| Nitrobenzene (NB), a hyper-toxic chemical, is one of 58 priority controlling pollutants of China and is on the list Environmental Priority Pollutant. It widely applies in the manufacture of agricultural, fuel, detonator, medicine, multimer and other chemical production. NB in environment which is mainly from industrial effluents during the manufacture of NB and aniline can pollute water body. Bio-accumulation, elimination of Nitrobenzene in carassius auratus and cyprinus carpio was studied under semi-static conditions, and used 0.5mg/L and 3.0mg/L nitrobenzene aqueous solution to simulate contaminated water environment. Gas chromatograph was applied to detect nitrobenzene in fish. Used homogenate and ultrasonic extraction in sampling preparation. This method is fast, high-efficient and stable. The recovery rate of sample is high. Used self-made glass column which is with alumina and anhydrous sodium sulfate in it to purifying the samples. It was indicated that the accumulation of different tissues in 0.5mg/L level was the same as which in 3.0mg/L level. Branchia and intestine absorbed NB fast and more. The maximum of NB were 15.21mg/kg in carassius auratus branchia and 13.41mg/kg in cyprinus carpio branchia. NB in muscle had multiple distribution. The curves of liver and kidney were different from curves of other tissues. Abnormal peaks proved indirectly that there was metabolic transformation of nitrobenzene in liver and kidney. Heart had the maximum in the 2.5th day, the became stabilization. The BCF in 0.5mg/L level was higher than that in 3.0mg/L level. The BCF values of carassius auratus tissues with 3.0mg/L level were: branchia 2.35, intestine 2.30, muscle 1.60, liver 1.61, kidney 1.95, heart 1.34. With 0.5mg/l they were: branchia 5.06, intestine 4.32, muscle 3.04, liver 3.42, kidney 3.80, heart 2.60. The BCF values of carps tissues with 3.0mg/L level were: branchia 1.54, intestine 1.45, muscle 1.33, liver 1.40, kidney 1.44, heart 1.33. With 0.5mg/L they were: branchia 4.22, intestine 4.14, muscle 2.44, liver 2.52, kidney 3.36, heart 2.42. The time when appeared maximum in 0.5mg/L level is fast that in 3.0mg/L level. In the same level carassius auratus absorbed NB much more than cyprinus carpio. When NB in fishes reached equilibrium(4.5d) in 0.5mg/L level, the carassius auratus was used to do release test. When the test began NB in all the tissue released fast. The rates of elimination in two kinds of fish were quick and accorded with first order kinetics equation, c = c0e-k·t ,and the correlation coefficients were 0.759 to 1.832. The half-life periods were: branchia 13.0 h, intestine 16.7 h, muscle 14.6 h, liver 17.9 h, kidney 17.4 h, heart 19.0 h. All kinds of tissues had different cumulant values. The results showed that the distribution of nitrobenzene in different tissues followed the rule: muscle>branchia>intestine>kidney>liver>heart. And the carassius auratus and cyprinus carpio followed the same rule.
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