Preparation Of A New Hernia Mesh Based On The INT6-HIF2a-PSPH Promoting Angiogenesis Mechanism

The recurrence rate of hernia is high,but the mechanism is unknown.This study believes that the main reason for hernia recurrence is that the hernia patch cannot be degraded.The specific manifestation is that the patch is wrapped in fibrous connective tissue in the body,forming a scar,resulting in insufficient vascularization during tissue repair,difficult for cells to infiltrate.Therefore,the purpose of this study is to prepare a new type of composite patch.The first part of this project was to screen the key genes for the recurrence of hernia through bioinformatics methods.The results of the study found that compared with normal muscle tissues,PSPH gene was higher expressed in hernia recurring tissues.The second part was to study the role of INT6/HIF2a/PSPH in angiogenesis.Bioinformatics suggested that INT6/HIF2a/PSPH was closely related to angiogenic factors.Cell experiments proved that after lentiviral vector interferes with INT6,PSPH expression was inhibited,HIF2a expression rose under normoxia,VEGFa expression rose as well.The third part of this project was to study the pro-vascularization mechanism of INT6/HIF2a/PSPH to prepare a composite hernia patch with triple slowrelease function of lentivirus.The structure of the composite patch was as follows:gelatin hydrogel was injected on the front of the decellularized material,and chitosan microspheres carrying siRNA-INT6 lentivirus were dispersed in the hydrogel;poly caprolactone was sprayed on the back of the decellularized material.In vitro studies showed that the composite patch had no cytotoxicity.Animal experiments found that the composite patch showing good histocompatibility,could promote angiogenesis and inhibit fibrous connective tissue proliferation.