The Combination Study On Nature And Flavor Split Component Of Rhizoma Anemarrhena

The drug property theory of Chinese Medicine is the essential components of Chinese Medicine System,the core of Chinese Medicine Theory system and the foundation for guiding clinical medication of Chinese Medicine.The nature and flavor including four kinds of nature and five kinds of flavor is the basis of drug property theory and the most valuable parts.On the basis of the hypothesis proposed of my tutor in this dissertation:"The hypothesis of scientific connotation on Chinese herbal medicine flavor and nature".The research team carried out theoretical research on the medicinal properties of Chinese herbal medicine for the efficacy of alleviating water.Anemarrhena asphodeloides is the drug that it’ s efficacy of alleviating water retention has been researched here and the research object in this dissertation.Anemarrhena asphodeloides’ nature is cold and its flavor is bitter and sweet,and it belonged to lung,stomach and kidney viscera in Chinese Medicine,furthermore,the function of Anemarrhena asphodeloides is clearing heat,purging fire,nourishing Yin and moistening dryness.Bitter and sweet medicine efficacy of Anemarrhena asphodeloides is taken separately in classical prescription of Baihu Decoction and Zhibaidihuang Pill,moreover,the flavor components and flavor of Rhizoma Anemarrhenae split components being combined to study is carried out to verify what the nature and flavor of split components belong to,clarify its effect and mechanism,which lays the theoretical foundation for the clinical fields.The research on the model of separation and combination of Chinese medicinal herbs’ nature and flavor should be improved further,in order to enrich the theories of Chinese medicinal herbs’ nature and flavor,and provide important reference for clinical application of drugs and development of new drugs.1.Literature ResearchDevelopment of Chinese drug property theory,research status of the main chemical composition and pharmacological action of Rhizoma Anemarrhenae,the classic Baihu Decoction and Zhibaidihuang Pill of prescription compatibility is discussed and main pharmacological and clinical effects were reviewed,the flavor of composability research development has laid a solid foundation for literature.Especially in doctor compatibility,summed up in the Baihu Decoction,the bitter flavor for clearing heat and purging fire effect and anemarrhena asphodeloides in Zhibaidihuang Pill with the effect of nourishing yin and tonifying kidney,its flavor’combination study provides a theoretical basis.2.Chemical characterization study on the material basis of Rhizoma Anemarrhenae’s nature and flavorEstablished in this paper,based on the early stage of the group split method based material base of Anemarrhena asphodeloides was split into four components,namely the polysaccharide components,components of ethyl acetate,saponin constituents and the rest of the components;The methods of HPLC-ELSD and UPLC-MS were used to analyze the chemical characteristics and verify their non-intersections.By silica gel,ODS,Sephadex column chromatography method and HPLC technology such as LH-20,the separation of ethyl acetate part a and part saponins of purification,a total of 24 compounds were isolated,according to the physical and chemical analysis,1D and 2D-NMR spectroscopy and mass spectrometry testing means such as a comprehensive analytical(guide)set of the chemical composition of the structure.The structure of 22 compounds was identified,including 13 saponins,6 flavones and 3 other compounds.3.Combination study of split group on Rhizoma Anemarrhenae’s nature and flavor in Baihu Decoction(1)Study on antipyretic effect of split components of Anemarrhena flavor in Baihu DecoctionIn order to reflect the theory of traditional Chinese medicine in compound Baihu Decoction of Rhizoma Anemarrhenae bitter efficacy as the research object,select the thermal model and the main clinical application,namely four thermal model,2,4 and two nitrophenol,dried yeast,lipopolysaccharide,bacterial endotoxint,study of flavor components,flavor of Rhizoma Anemarrhenae split components instead of the combination of Anemarrhena in Baihu Docotion,the lack of Anemarrhena baihutang group,bitter component(saponin component+polysaccharides+other components)+Anemarrhenae baihutang group,sugariness missing components(ethyl acetate layer)+baihutang,lack of Anemarrhenae polysaccharide fractions+lack of Baihu Decoction,Anemarrhena saponin fraction,the lack of baihutang+Anemarrhenae component missing white tiger Soup+Anemarrhenae antipyretic effect,the results showed that:in the experiment of pharmacology reflected in Baihu Decoction of Rhizoma Anemarrhenae bitter,bitter flavor is an effective component group of Baihu Decoction of Rhizoma Anemarrhenae antipyretic,can replace the raw materials and corresponding compatibility of Rhizoma Anemarrhenae.Split group with bitter flavor of antipyretic effect of saponin and polysaccharide,which play a better role of cooling,the remaining group also has curative effect,but the treatment effect is lower than that of bitter components,further validation of Anemarrhena taste split components belonging,that can be combined the taste of the split group,confirmed the theory of the scientific connotation hypothesis of Chinese herbal flavor.(2)Mechanism study of split group on Rhizoma Anemarrhenae’s nature and flavor in Baihu DecoctionIn Baihu Decoction of Rhizoma Anemarrhenae flavor split component research on combination line four thermal experiment,observe the heating factor is TNF-α,IL-6,cAMP,IL-1β,PGE2 and cAMP/cGMP in the blood and hypothalamus inhibition,and the expression in parts of the lung meridian TNF-α,IL-6,IL-1β factor,found and taste of Rhizoma Anemarrhenae split components of multi-channel and multi targets play antipyretic effect,models of different drug groups the inhibitory factor of different fever.Correlation analysis and components through the heating factor expression,Anemarrhena bitter component and bitter split components mainly through blood and lung in TNF-α,IL-1β factor and hypothalamic cAMP,PGE2 play a role in antipyretic,bitter component can replace the raw materials and to Rhizoma Anemarrhenae combined with the corresponding role,mainly play a bitter taste the active ingredients are saponins and polysaccharides,this mechanism results which further verify the antipyretic,Anemarrhena taste split components can be combined and Anemarrhena bitter component can replace the inherent advantages of the compound of Rhizoma Anemarrhenae,provide an important basis for the scientific flavor of the combination of Chinese medicine and property theory hypothesis(3)Combination study of split group on Rhizoma Anemarrhenae’s nature and flavor in Baihu DecoctionPrototype components and metabolites in the blood detected by UPLC-MS sugariness compound group and bitter compound group after administration,found 1 prototype components,The of methods PCA and OPLS-DA were analyzed before and after administration of compound bitter and bitter compound of blood and urine metabolites in the blood profile,metabolism,analysis method of PCA and OPLS-DA effect of Rhizoma Anemarrhenae sweet components and bitter components of metabolic differences,two distinct effects on the metabolite,showing the effects of different taste of metabolites the different changes in the urinary metabolites,although the sweet and bitter compound of compound group and non group compound Rhizoma Anemarrhenae metabolites have distinguishing trend,but no significant distinction between the two,but there is speculation separation trend,sweet component does not play a role.The compounds in the bitter compound group and the compound of the non Anemarrhena compound prescription were well differentiated.It was speculated that the bitter component is the main component of the compound in the compound prescription.Flavor components of Rhizoma Anemarrhenae instead of Anemarrhena combination Baihu Decoction metabolism study,re-verify the bitter components in Rhizoma Anemarrhenae in Baihu Decoction played an obvious role,and sugariness group did not obviously play a role,which is consistent with the Baihu Decoction compound take function of Anemarrhena bitter flavor of clearing heat and purging fire,described in the compound is different in nature and flavor of drugs the sweet ingredients and most bitter components play a role,It confirmed the hypothesis of scientific connotation on Chinese herbal medicine flavor and nature.4.Combination study of split group on Rhizoma Anemarrhenae’s nature and flavor in Zhibaidihuang Pill(1)Combination study on split group of Rhizoma Anemarrhena’s nature and flavor in the treatment of nephritis in Zhibaidihuang PillStudy on the flavor components of Rhizoma Anemarrhenae combined therapeutic effect of Zhibaidihuang Pill on nephritis,6 biochemical and histological observation of pathological changes of flavor components of Rhizoma Anemarrhenae split treatment,the results showed that:in the experiment of pharmacology reflected in Zhibaidihuang Pill Rhizoma Anemarrhenae,sweet in nature and flavor is an effective component group in Zhibaidihuang Pill with the effect of nourishing Yin and tonifying kidney,can replace the raw materials and corresponding compatibility of Rhizoma Anemarrhenae.Split group with bitter flavor of antipyretic effect of saponin and polysaccharide,which play a better role of cooling,the remaining group also has curative effect,but the treatment effect is lower than that of bitter components,to further verify the nature and flavor of split components of attribution,and to provide an important basis for the science of studying the combination of nature and flavor and flavor of the medicine theory hypothesis.(2)Combination study on mechanism of split group on Rhizoma Anemarrhenae’s nature and flavor in Zhibaidihuang PillTo observe Nephritis Models of rats after administration of deposition in kidney of antigen antibody complex and renal immunohistochemistry TNF-α,IL-6,IL-1βexpression of inflammatory cytokines,which can replace the sugariness components of raw materials and to according compatibility of Anemarrhena,this mechanism research results and purging heat effect results are basically consistent.To further validate the nature and flavor of Anemarrhena split components combined and Anemarrhena bitter component can replace the inherent advantages of the compound of Rhizoma Anemarrhenae,to provide an important basis for the science of studying the combination of nature and flavor and flavor of the medicine theory hypothesis.The effect of inhibition of related factor expression in renal tissue of Anemarrhena effective group can also nature and flavor resolution,scientific verification of Rhizoma Anemarrhenae by the kidney,and once again proved the Anemarrhena sugariness nourishing Yin and tonifying kidney in Zhibaidihuang Pill.(3)Combination study of split group on Rhizoma Anemarrhenae’s nature and flavor in Zhibaidihuang PillIn the blood metabolites detected by UPLC-MS sugariness compound group and bitter compound group after administration,the metabolite of bitter sweet flavor compound group and compound group,was significantly lower in its content,that bitter components play a lipid-lowering effect through the compound effect.Methods PCA and OPLS-DA were analyzed before and after administration of compound bitter and bitter compound of blood and urine metabolites in the metabolic profile,hematuria,analysis method of PCA and OPLS-DA effect of Rhizoma Anemarrhenae sweet components and bitter components of metabolic differences,two distinct effects on the metabolite,show the effects of different nature and flavor of metabolites different,different nature and flavor is sweet again validation of drug ingredients and most bitter components play a role that "The hypothesis of scientific connotation on Chinese herbal medicine flavor and nature" the flavor of the medicine theory.Through the LysoPC metabolites in the bitter aftemature and flavor of Baihu Docotion and the content change of compound bitter afternature and flavor Zhibaidihuang Pill,found that the same drug in different compound,different flavor compound helps,but also further elaborate the theory of Chinese medicine in different drug guide with official medicine scientific theory play different roles,to provide the reference for science study on the theory of Chinese medicine.The innovation and characteristics of this research1.This paper is based on the Chinese medicine(gas)flavor scientific connotation hypothesis,guided by Chinese medicine theory,research the flavor components of Rhizoma Anemarrhenae recombination,in order to reflect the different nature and flavor,Baihu Docotion and Zhibaidihuang Pill as the research object,experimental method the establishment of therapeutic effects mechanism of metabonomics three level verification of nature and flavor components belonging to split,to clarify the"theory of compatibility effect is related to nature and flavor",to provide scientific basis for the study of combination.2.For the first time,split group of nature and flavor instead of combination of compound Rhizoma Anemarrhenae as the research object,pharmacological effect and mechanism of Chinese medicine of various flavor components in the compound,found the bitter taste of Anemarrhena playing the role of clearing heat and purging fire in Baihu Docotion,Anemarrhena sugariness efficacy of nourishing yin and tonifying kidney in Zhibaidihuang Pill,illustrates the significance and role prescription compatibility,objectively further demonstrates the new hypothesis of the theory of Chinese medicine property.3.The results of the study is based on the treatment of nephritis antipyretic and sugariness Compound Rhizoma Anemarrhenae bitter compound group Anemarrhena components in Baihu Docotion in the Zhibaidihuang Pill in the treatment based on characteristics of TCM syndrome differentiation,to provide a scientific basis for exploring the combination of prescription for clinical use and provide new ideas for further improvement of traditional Chinese medicine the theory of compatibility.