Regulation On Yield And Dry Matter Partioning Of Cistanche Tubulosa(Schenk)Whight

The main problems facing the artificial cultivation of Cistanche tubulosa (Schenk) R. Wight are the low level of production and inadequate cultivation measures, which seriously affect the producting and marketing of C.tubulosa and the associated products. In this study, using Tamarix L and C.tubulosa as materials, we discussed the law of growth and development of Tamarix L, source-sink relationship and the law of production and allocation of non-structural carbohydrates of Tamarix L and C.tubulosa, and regulated substances allocation betweent Tamarix L and C.tubulosa through appropriate cultivation measures, all of which provided a theoretical basis for the high-yielding and standardized cultivation of C.tubulosa. The main conclusions are as follows:1.1Age Tamarix plant had a dry weight of141.6g, the allocation ratio of which gradually decreased for stems, while increased for leaves and roots. The content, accumulation and allocation ratio of non-structural carbohydrate significantly improved in roots of Tamarix, which indicated that more non-structural carbohydrates were transported and allocated to the roots of the autumn Tamarix.2. Dry matter of Tamarix plant significantly increased from age1to age4, which reached as high as2297.7g by age4. With increasing age, the content, accumulation and allocation ratio of non-structural carbohydrate significantly improved in the roots of Tamarix, which indicated that more dry matter were transported and allocated to the roots as the age increased.3. Dry weight of Tamarix parasited by C.tubulosa wasless than the Tamarix not parasiticd by C.tubulosa, reaching127.3g on November1st, the allocation of which gradually increased in C.tubulosa. The content, accumulation and allocation ratio of non-structural carbohydrate significantly improved in C.tubulosa, which indicated that the original allocation pattern of Tamarix had been broken after parasited by C.tubulosa, resulting in more non-structural carbohydrates transported and allocated to the C.tubulosa.4. C.tubulosa parasitized Tamarix of different ages, the total dry weight of Tamarix and C. tubulosa reached the highest of4186.6g when the host Tamarix was of age5. Non-structural carbohydrate content of C.tubulosa decreased, while the accumulation increased significantly, which indicated that increased age of Tamarix namely increased source, to help improve the production of C.tubulosa.5. With increasing amount of parasites C.tubulosa, plant height and crown diameter of Tamarix reduced, while stem diameter increased, and dry weight of Tamarix-C.tubulosa increased significantly. Non-structural carbohydrate content of C.tubulosa decreased, while the allocation ratio improved significantly. It indicated that increased amount of parasites C.tubulosa namely stronger sink, which enhanced the ability of C.tubulosa to get non-structural carbohydrates, leading more carbohydrates transported and allocated to C.tubulosa.6. Nitrogen can regulate the yield and quality of C.tubulosa by regulating the substance synthesis ability of Tamarix and the strength of the sink C.tubulosa. Appropriate amount of nitrogen fertilizer increased the chlorophyll content and net photosynthetic rate of Tamarix, which improved the ability of substance synthesis, increased the assimilates supply for C.tubulosa and improved the dry weight of C.tubulosa; Enhanced activity of enzyme related to glucose metabolism in C.tubulosa resulted in more non-structural carbohydrates transported to C.tubulosa, thereby increased the content of active ingredient in C.tubulosa.7. Paclobutrazol can improve the yield and quality of C.tubulosa by increasing the substance synthesis activity of Tamarix and regulating the substance allocation between Tamarix and C.tubulosa. Appropriate concentration of paclobutrazol improved the chlorophyll content and the net photosynthetic rate of Tamarix and inhibited the growth of Tamarix, so that more assimilates were transported to C.tubulosa, to increase the dry weight and the content of non-structural carbohydrate in C.tubulosa, thereby increased the content of active ingredient in C.tubulosa.8. Moderate topping significantly increased the allocation ratio of dry weight for C.tubulosa, and also the contents and accumulation of carbohydrates in C.tubulosa plant, thereby increased the content of active ingredient in C.tubulosa.