| Cistanches Herba is the dry fleshy stem with scales of Cistanche deserticola(Cistanche deserticola Y.C.Ma)and Cistanche tubulosa(Cistanche tubulosa(Schenk)Wight).The host of C.deserticola is the Chenopodiaceae plant[Haloxylon ammodendron(C.A.Mey.)Bunge],while the host of C.tubulosa is the(Tamarix)plants.At present,the Cistanches Herba mainly comes from artificially cultivated varieties.Due to the short cultivation history and severe germplasm differentiation,especially the obvious differentiation of stem and flower colors.At the same time,in the vaccination experiment,it was found that[Atriplex canescens(Push)Nut.]can also be inoculated with C.deserticola,and began to be promoted in the northwest region.In order to provide scientific basis for the inoculation of C.deserticola and the cultivation and purification of C.tubulosa,as well as for the selection of excellent germplasm,this paper uses the pharmacological components of "tonifying kidney yang,benefiting essence and blood" such as phenylethanoid glycosides(echinacoside,tubuloside A,verbascoside,isoverbascoside,2’-acetylverbascoside)and "moistening intestines and laxative" such as betaine,mannitol,fructose,glucose,and sucrose)as indicators,comparative analysis were the quality of C.deserticola parasitized at H.ammodendron and A.canescens from different origins,and the quality of C.tubulosa with different stem color types and growth patterns.The main research findings are as follows:1.An HPLC-UV quantitative analysis method for 5 phenylethanoid glycosides in Cistanche Herba(echinacoside,tubuloside A,verbascoside,isoverbascoside,2’-acetylverbascoside)was established,and the linear relationship of the five components was good(R2≥0.9998),the precision,stability,and repeatability RSD were all less than 3.75%,and the sample recovery rate was 97.50%-99.52%(RSD<2.79%).Based on HPLC-ELSD,a quantitative analysis method for betaine,mannitol,fructose,glucose,and sucrose was established.The methodological investigation results showed that the precision,repeatability,and stability RSD were all less than 2.95%,and the sample recovery rate was 96.29%-99.78%(RSD<2.22%),meeting the requirements of quantitative methodology.2.There are differences in the content of medicinal components in C.deserticola parasitized at H.ammodendron and A.canescens.The average total contents of 5 kinds of phenylethanoid glycosides in A.canescens-C.deserticola is 2.85%,which is significantly higher than that in H.ammodendron-C.deserticola(1.56%);the sum content of betaine,fructose,mannitol,glucose and sucrose in A.canescens-C.deserticola is 55.12%,higher than H.ammodendron-C.deserticola(45.69%),but there was no significant difference.3.There are differences in the contents of five phenylethanoid glycosides,betaine,mannitol,fructose,glucose,and sucrose in H.ammodendron-C.deserticola and A.canescens-C.deserticola from different origins.The total content of 5 kinds of phenylethanoid glycosides in H.ammodendron-C.deserticola was 1.84%,which was the highest of Gansu,followed by 1.44%and 1.45%of Xinjiang and Inner Mongolia respectively;five kinds of betaine,mannitol,fructose,glucose and sucrose total content in H.ammodendron-C.deserticola was 49.60%of Gansu,significantly higher than 46.71%of Inner Mongolia and 41.63%of Xinjiang.The total content of 5 kinds of phenylethanoid glycosides in A.canescens-C.deserticola is the highest in Gansu,which was 4.28%,which was significantly higher than 2.07%of Xinjiang and 1.82%of Inner Mongolia;the sum content of betaine,mannitol,fructose,glucose,and sucrose was highest of Inner Mongolia is 63.84%,which significantly higher than 50.53%of Gansu and 46.85%of Xinjiang.4.The relationship between different stem color types of C.tubulosa and the content of medicinal components was clarified.The total content of five kinds of phenylethanoid glycosides in purple C.tubulosa at the upper part of the stem was 9.78%,which was significantly higher than the 6.33%of the white at the upper part of the stem;the sum content of betaine,fructose,mannitol,glucose and sucrose is 13.86%of the upper part of the purple stem,which is not different from the 13.51%of the upper part of the white stem.The total content of the five phenylethanoid glycosides in brown yellow C.tubulosa at the lower part of the stem was 11.10%,which was higher than that of the purple and white at upper part of the stem,and there are significant differences with the white at the upper part of the stem,but the sum content of fructose,mannitol,glucose,and sucrose was 8.65%,which was significantly lower than the content of purple and white types at the upper part of the stem.5.There was no significant difference in the content of phenylethanoid glycosides between the fleshy stems grown from inoculation of the C.tubulosa seed and the fleshy stems grown from parasitic disk,but the former was significantly higher than the latter in terms of sugar alcohols.The total content of the five phenylethanoid glycosides in the fleshy stems grown from the parasitic disk of C.tubulosa was 10.80%,and the total content of the fleshy stems grown from seeds inoculated was 8.77%;the sum of fructose,mannitol,glucose,and sucrose in the fleshy stems grown from seeds inoculated was 19.37%,which was significantly higher than 12.68%of the fleshy stems grown from parasitic disk.6.There are significant differences in the content of medicinal components between C.deserticola and C.tubulosa.The total content of five phenylethanoid glycosides in C.tubulosa was 8.05%,which was significantly higher than 2.01%in C.deserticola.Five components of betaine,fructose,mannitol,glucose and sucrose were detected in C.deserticola,the content was 5.93%,8.07%,5.89%,5.92%,23.06%,respectively;fructose,mannitol,glucose and sucrose were detected in C.tubulosa,the content was 5.11%,5.71%,3.51%and 1.21%,respectively,but betaine was not detected,and the content of fructose,glucose and sucrose in C.deserticola were significantly higher than those in C.tubulosa.Conclusion:A method for the determination of five phenylethanoid glycosides in Cistanches Herba was established,and HPLC-ELSD quantitative analysis of 5 components of betaine,fructose,mannitol,glucose and sucrose.The high quality host of C.deserticola is A.canescens,and Xinjiang,Gansu and Inner Mongolia are all suitable production areas of C.deserticola.It was revealed that the differences in the content of active ingredients in different stem colors and growth patterns of C.tubulosa,and the purple at the upper part of the stem and the brownish yellow at the lower stem can be used as the optimal germplasm of C.tubulosa.The differences in the effective components of C.deserticola and C.tubulosa were clarified. |
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