Study On Anti-EV71Effects And Influences On MIR-146a, MIR-155and TLR4mRNA’s Expressions Of EV71’s Infection Of Ganlu Xiaodu Dan And Its Incomplete Prescriptions In Vitro

Backgrounds The epidemic situation of hand-foot-mouth disease is getting severer; it is immediacy to find effective ways to treat it. Ganlu Xiaodu Dan is an effective prescription for hand-foot-mouth disease, but the laboratory studies of Ganlu Xiaodu Dan is inadequate, so the mechanism of it still remains unknown.Objective To explore the anti-EV71effects of Ganlu Xiaodu Dan and its incomplete prescriptions, and explore the regulation and control effects of Ganlu Xiaodu Dan and its incomplete prescriptions in the inflammation and immune injury that induced by EV71,then recognize the scientific connotation of Ganlu Xiaodu Dan’s structure further.Mothods1) Literature research:To discuss Ganlu Xiaodu Dan’s effectiveness on epidemic disease from its resource, mechanism of prescription, the situation of its clinical application and its pharmacological action.2) With help of cell culture to observe the anti-EV71effects of Ganlu Xiaodu Dan, and its incomplete prescriptions of Qing prescription and Li prescription, in inhibiting-virus-directly experiment, therapeutic-inhibiting-virus experiment, preventive-inhibiting-virus experiment and preventive-therapeutic-inhibiting-virus experiment.3) To observe changes of expressions of miRNA-146a, miRNA-155and TLR4in RD cells in inhibiting-virus-directly experiment with intervention of Ganlu Xiaodu Dan, Qing prescription and Li prescription by method of RT-qPCR.Results1) Ganlu Xiaodu Dan was created by Ye Tianshi, first recorded in Xu Ming Yi Lei An. It can be used in treating various clinical diseases, most in internal medicine diseases and infectious diseases. The diseases which be treated most frequently are viral hepatitis, chronic gastritis and hand-foot-and-mouth disease. It can protect liver, anti liver fibrosis, regulate the immune and lipid metabolism and gastrointestinal function, and it has antiviral, anti-inflammatory, antipyretic effects as well. Those clinical uses and experimental studies of Ganlu Xiaodu Dan comfirmed its effectiveness on epidemic disease, the epidemic pathogenic factor and damp and hot is the important syndrome’s pathological basis of epidemic disease which Ganlu Xiaodu Dan can treat.2) In inhibiting-virus-directly experiments, therapeutic-inhibiting-virus experiment and preventive-therapeutic-inhibiting-virus experiment, groups of Ganlu Xiaodu Dan, Qing prescription and Li prescription had higher cell survival rate and virus inhibition rate than group of ribavirin. Group of Qing prescription had higher cell survival rate and virus inhibition rate than groups of Ganlu Xiaodu Dan and Li prescription. In preventive-inhibiting-virus experiment, cell survival rates of groups of Ganlu Xiaodu Dan, Qing prescription, Li prescription and ribavirin were close to that of the virus control group, and their virus inhibition rates were almost zero.3) Ganlu Xiaodu Dan and its incomplete prescriptions could elevate levels of miRNA-146a and miRNA-155, which dropped when cells infected with EV71. Li prescription’s regulatory effect was better than Ganlu Xiaodu Dan and Qing prescription.Conclusions1) Ganlu Xiaodu Dan is a good prescription for epidemic disease, and it is worthy spreading in clinic. The epidemic pathogenic factor and damp and hot is the important syndrome’s basis of epidemic disease that Ganlu Xiaodu Dan can treat.2) Ganlu Xiaodu Dan and its incomplete prescriptions have good antiviral effects of EV71, and they can affect multiple links of EV71’s multiplication.3) Heat-clearing and toxin-relieving therapy has stronger anti-EV71effect, but the effect of therapy of removing damp and turbid can not be ignored, they can supplement each other.4) Ganlu Xiaodu Dan can regulate the inflammatory and immune responses which induced by EV71by regulating miR-146a,miR-155and TLR4, which indicates that in addition to direct-anti-EV71effect, Ganlu Xiaodu Dan has an indirect effect of anti-EV71by ways of regulating inflammatory and immune responses. It is worth promoting in treating hand-foot-mouth disease in clinic.5) Heat-clearing and toxin-relieving therapy has stronger anti-EV71effects than therapy of removing damp and turbid, but therapy of removing damp and turbid has stronger effect in regulating inflammatory and immune responses that induced by EV71.This indicates that whether Heat-clearing and toxin-relieving therapy or therapy of removing damp and turbid has its strong points, and each of them can not be deleted at random.6) Whether as for direct-anti-EV71or as for regulating inflammatory and immune responses, herbs of heat-clearing and toxin-relieving therapy and herbs in therapy of removing damp and turbid in Ganlu Xiaodu Dan may have some effects of mutual antagonism, this need to be studied further.