Effect And Mechanism Of Compound Chuanji Formula In Treatment Of Vertebral Artery Type Of Cervical Spondylosis In Rabbit Model

ObjectiveFirst of all:to rebuild the rabbits model of vertebral artery type of cervical spondylosis and explore the efficacy compound chuanji formula in treatment of rabbits model of cervical spondylosis. The second:to detect the expression of MMP-2 andα-SMA in the vertebral artery and the interference effects of compound chuanji formula to it. The third:to interpret the pathogenesis of the vertebral artery in vertebral artery type cervical spondylosis and develop the mechanism of Chinese medicine in treatment of vertebral artery type of cervical disease.MethodsForty rabbits were randomly divided into four groups:the normal group, the control group, the Jingfukang group (JFKG) and the chuanji formula group (CJFG). The method of injecting sclerosing agent was used to induce the vertebral artery type of cervical spondylosis animal model.10 mL scleroing agent was injected into the left side of C3 to C5 cervical vertebra next to the edge of transverse process with the syringe needle. The above steps were repeated at the 1st day of the 2nd and 4th week, respectively. Four weeks later, the TCD detection showed that the pulsatility index, resistent index were significantly higher and the blood flow rate was remarkedly slower in the left side vertebral artery than those in the right side vertebral artery in the control group, JFKG and CJFG respectively (p<0.05); Brainstem auditory evoked potentials (BAEP) showed thatⅢ,Ⅴlatencies were longer than four weeks ago in the control group, JFKG and CJFG (p<0.05). It showed the animals model were successfully reproduced.The control group, JFKG and CJFG were treated with 20 ML Normal saline,20 ML suspended compound chuanji formula or 20 ML suspended Jingfukang granule respectivily with intragastric administration, once a day. After four weeks therapy, the transcranial doppler was used to detect the blood flow rate,pulsatility index and the resistent index of the vertebral artery;BAEP apparatus detected theⅠ,Ⅲ,Ⅴlatency; the blood flow rheometer was. used to detected the plasma viscosity and the blood viscosity; the HE staining was used to measure the thickness of tunica media vasorum, the immunohistochemistry was used to detect the expression ofα-SMA and the expression of MMP-2 in the vertebral artery.ResultsThe plasma viscosity, blood viscosity, pulsatility index, resistent index, thickness of tunica media vasorum, expression ofα-SMA and the expression of MMP-2 were significantly higher in the model group than those in the normal group, theⅢ,Ⅴlatency was significantly longer (p＜0.05), in the contrast, the blood flow rate was remarkably decreased (p＜0.05). The plasma viscosity, blood viscosity, pulsatility index, resistent index, thickness of tunica media vasorum, expression ofα-SMA and the expression of MMP-2 were decreased significantly in the CJFG compared with the normal group and the control group (p<0.05), and the blood flow rate was increased remarkably (p<0.05) Compared with the control group, theⅢ,Ⅴlatency was significantly longer in the CJFG (p＜0.05); Compared with the JFKG, theⅢ,Ⅴlatency was the samilar (p>0.05).TheⅠlatency was not changed too much in the four group (p＞0.05)ConclusionCompound chuanji formula is effective in treatment of the animal model of vertebral artery type cervical spondylosis; The tunica media vasorum proliferation is one of the mechanism of blood-supply insufficient in vertebral artery type of cervical spondylosis; It would improve the blood supply to the brain stem, decrease the plasma viscosity, blood viscosity, pulsatility index and resistent index, also increase the blood flow rate. Proliferation and reconstitution of vertebral artery is one of the mechanism of vertebral artery type of cervical spondylosis; CJP is effective in inhibit the proliferation and reconstitution of vertebral artery.