| This dissertation includes two parts, i.e. literature research and animal experiment. Literature researchDelayed-onset muscle soreness (DOMS) describes muscle pain and tenderness that typically develop several hours postexercise and consist of predominantly eccentric muscle actions, especially if the exercise is unfamiliar. As we know, traditional Chinese Tuina is widely used in sports teams with its unique advantages in relieving exercise-induced fatigue and strengthening sporting abilities, and it has gained more and more attention in prevention and treatment of DOMS. This part reviews the relative literatures in western and traditional medicine, and sums up the pathogenesis and evaluation of DOMS, and approaches to prevention and treatment for DOMS. This part also covers the mechanism of Tuina on preventing and treating sports injuries, which provides theoretical basis for prevention and treatment on DOMS by Tuina. The design of animal experiment, observing data and experimental method were established on the basis of these literatures.Animal experiment researchObjective: to evaluate the effects of Tuina on DOMS and the relationship of oxidative-antioxidative balance disturbance in rats by testing serum LD, LDH, CK, MDA,SOD,·OH.Method130 healthy and clean male SD rats of six-week old were randomly divided into 4 groups including normal control group(A), exercise control group(B), Tuina pre-exercise group(C) and Tuina post-exercise(D). Group B, C and D were randomly divided into 1h, 12h, 24h and 48h groups. Each group consists of 10 rats. Group B were put into a glass pool of 100 cm×60 cm×70 cm to swim carrying a subject of 3% its body weight till exhausted. Group C and Group D were treated 10 minutes by Tuina pre-exercise or post-exercise. The rats in Group B, C and D were killed to take blood sample 1h, 12h, 24h and 48h post-exercise or after Tuina. While the normal control group were killed after 48h to take blood sample.Results(1) In comparison with the normal control group, the level of serum LD in exercise control group, Tuina pre-exercise group and Tuina post-exercise rose significantly. In comparison with the exercise control group, the level of serum LD in Tuina pre-exercise group and Tuina post-exercise lowered significantly. (2) The level of serum LDH rose immediately to peak after 12h postexercise. The level of serum LDH lowered in level with LD in Group B, C and D. (3) In comparison with the normal control group, the level of serum CK in group B, group C and group D rose immediately. In comparison with group B, the level of serum CK in 1h, 12h in group C had no significant difference while it rose significantly in 1h, 12h in group D. In comparison with group C, the level of serum CK after 1h,12h and 48h in group D rose signifieantly. (4) The level of serum·OH rose immediately to peak after 1h in group B. In comparison with group B after 1h,12h, the level of serum·OH lowered signifieantly in group C and group D. The level of serum OH in 1h, 12h, and 24h had no significant difference between group C and group D. (5) The level of serum MDA rose signifieantly in group B. In comparison with group A, the level of serum MDA in 1h,12h and 24h in group C had no significant difference. While the level of serum MDA after 12h, 24h and 48h in group D had no significant difference. (6) In comparison with group A and group B, the level of serum SOD rose signifieantly in 1h, 12h, 24h and 48h in both group C and group D. (7) In comparison with group A, the ratio of SOD/MDA lowered significantly in group B. In comparison with group B, the ratio of SOD/MDA rose signifieantly in 1h, 12h, 24h and 48h in both group C and group D.Conclusion(1) The change of serum LD is related with exercise-induced fatigue, but it is not related with DOMS. (2) Tuina pre-exercise is effective in alleviating muscle damage caused by eccentric exercise in DOMS. (3) Tuina pre-exercise or postexercise are effective in lowering the level of serum OH and MDA, while raising the activity of serum SOD and the ratio of SOD/MDA. Tuina pre-exercise is more effective. (4) This experiment focused on Tuina pre-exercise or postexercise on rats of DOMS in testing the changes of LD,LDH,CK,·OH,MDA and SOD. The experimental result shows that Tuina pre-exercise is effective in lowering the level of serum LDH and CK. Tuina pre-exercise or postexercise are effective in lowering the level of serum·OH and MDA, while raising the activity of serum SOD and the ratio of SOD/MDA. Tuina is effective in delaying DOMS, reducing muscle soreness, controlling the damage of lipid peroxidation, increasing the ability in anti-oxidation and has good protection to muscle cell. |
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