Acupuncture Combined With Guli Granules Ovariectomized Rats Osteocalcin Metabolism

Objective:To investigate the combinative treatment of acupuncture and Gu-li' s affections on ovariectomized rat' s bone mineralization,hormones involved in the bone metabolism and the expression of BGP mRNAand BMP-2 mRNA as well,the research was designed to observe and compare the results of treatments for ovariectomized rats' osteoporosis by using acupuncture and moxibustion treatment,Gu-li,combination of acupuncture and moxibustion and Gu-li,nilestriol in the hope of providing an experimental basis for the combinative treatment of acupuncture and herb and an effective clinical treatment on osteoporosis.Method:Female SD rats of six months were established to osteoporosis model by removed the ovaries under anesthesia were randomized into model,acupuncture and moxibustion treatment,Gu-li,combination of acupuncture and moxibustion and Gu-li and nilestriol groups,acupuncture and moxibustion treatment group which received only acupuncture and moxibustion therapy and combination of acupuncture and Gu-li group which received acupuncture and moxibustion plus Chinese herb Gu-li powder therapy.In the two groups,bilateral "Dazhu","Shenshu","Pishu" and "Mingmen" were punctured with acupuncture needle and fumed with moxa stick once daily and 6 treatment periods altogether.Rats of Gu-li group were drenched with Gu-li powder once daily and nilestriol group was drenched with nilestriol once weekly.After trentment,blood samples were collected from femoral artery.(1)Bone calcium and phosphonium of the lumbar vertebra by using flame atomic absorption method and moly-stibine anti-colormetric method respectively.(2)Bone mineral density of the femur of the rats were detected by using dual energy X-ray bone density instrument and bone strength detected by three-spot bending test.(3)Structure-morph of femur cancellous bone trabecula was observed by sCanning electron microscope.(4) Serum E₂,PTH,CT,BGP were assayed by using radioimmunoassay.(5) Expression of BGP and BMP-2 gene were examined with the method of RT-PCR in bone tissue of osteoporosis rats.Result:After the rats had been removed the ovaries,bone calcium and phosphonium and bone density lost and nature of bone vitodynamics depressed obviously,shapes of bone trabecula were thinner and quantities were decreased,uterus weight depressed obviously,content of serum E₂,CT,BGP degraded and PTH advanced significantly,BGP and BMP-2 gene expression been weaker than sham group(P＜0.01).Metratrophia was controlled after treatment with the method of combination of acupuncture and Gu-li,acupuncture and Gu-li,serum E₂,CT,BGP raised obviously and PTH decreased moderately,BGP gene advanced and displayed positive correlation compared with serum BGP and BMP-2 gene heightened too,bone calcium and phosphonium and BMD and index bone vitodynamics increased at different degree.combination of acupuncture and Gu-li group surpassed significantly acupuncture treatment and Gu-li group from results of statistical analysis of effects on serum E₂,CT,PTH,BGP and BGP and BMP-2 gene and bone calcium and phosphonium,BMD and index bone vitodynamics(P＜0.05 or P＜0.01).Conclusion:(1)Model of osteoporosis was successfully duplicated. (2)Acupuncture and moxibustion,Gu-li powder,acombination of acupuncture and Gu-li can all improve and normalize bone matrix mineralization,improve bone microarchitecture and reinforce femoral bone strength.(3)Acupuncture and moxibustion,Gu-li powder,combination of acupuncture and Gu-li can all adjust the expression of hormone of E₂,PTH and CT which participated in bone calcium metabolism.(4)Acupuncture and moxibustion,Gu-li powder and combination of acupuncture and Gu-li can all promote the expression of ovariectomized rats' femur of BGPmRNA and BMP-2mRNA.(5) The treatment of acupuncture and moxibustion combined with Gu-li powde is obviously superior to single-method therapy of acupuncture or Gu-li.