The "double Solid Links" Mechanism Needling Method For Treatment Of Postmenopausal Osteoporosis Model Rats

Objective:To study the mechanisms that acupuncture therapy of "Shuanggu Yitong" can treat postmenopausal osteoporosis (PMO).To supply experimental evidence approving acupuncture can prevent and treat osteoporosis. Methods:Choosing female SD rats aged 6-10 months then resecting double ovaries and feeding them for 90 days in order to make the model of experimental osteoporosis. Then, dividing them into 5 groups such as control group, regular acupoints applying group, Shuanggu group, Yitong group, Shuanggu Yitong group, with 12 rats each group. There wasn't any therapy in control group; We applied Pishu(BL 20), Weishu(BL 21), Shenshu(BL 23), Qihaishu(BL 24) for the regular acupoints applying group; Guanyuan(RN 4), Zusanli(ST 36) for the Shuanggu group; and Shenshu(BL 23), Geshu(BL 17), Dazhu(BL 11) for the Yitong group; Guanyuan(RN 4), Zusanli(ST 36), Shenshu(BL 23), Geshu(BL 17), Dazhu(BL ll)for the Shuanggu Yitong group. Giving electroacupuncture therapy once a day, 15 minutes each time, holding the needles for 10 minutes. Then these rats rested for 1 day after consecutive therapy for 6 days. The therapy lasted for 8 weeks. (1) Using automatic serum analyzer to measure serum calcium (Ca), phosphate (P), alkaline phosphatase (ALP) and urinary calcium, phosphate, creatinine (Cr); radioimmunoassay to measure serum bone Gla-containing protein (BGP); chemiluminescent immunoassay to measure deoxypyridinoline (D-Pyr). (2) Using dual-energy X-ray absorptiometry to measure the rats' bone mass content (BMC) and bone mass density (BMD) of lumbar 1-5 and thighbone. (3) Using double antibody + PEG to measure serum estradiol (E2). (4) Using microscope to observe the morphological structure of bone trabeculae, applying VIDAS image analyzer system to measure %trabecular volume (TBV%), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp). (5) Applying ELISA to examine insulin-like growth factor-1 (IGF-1). (6) Using RT-PCR to detect the expression level of interleukin(IL)-6 mRNA in bone tissue. (7) Using RT-PCR to examine the expression level of osteoprotegerin(OPG) mRNA in bone tissue. Results:(1) The biochemical measurement indexes of bone metabolism indicate: the indexes of serum calcium, phosphate, BGP are not different significantly among 5 groups (P>0.05). Compared with control group, in the other four groups, serum ALP, urinary Ca/Cr, P/Cr all decreased (P<0.05), the indexes decreased significantly in Shuanggu Yitong group(P<0.01). The index didn't differ significantly among regular acupoints applying group, Shuanggu group, Yitong group (P>0.05). The urinary D-Pyr in all the acupuncture therapy groups decreased (P<0.05), and there were no statistic significance among acupuncture therapy groups. Conclusion: Acupuncturecan decrease serum ALP, urinary Ca/Cr, P/Cr of osteoporosis model rats without ovaries and "Shuanggu Yitong" acupuncture therapy superior to other regular acupoints applying acupuncture therapy.(2) The indexes of BMC and BMD measurement indicate: Compared with control group, in the other four groups, BMC and BMD all increased (P<0.05), BMC and BMD increased significantly in Shuanggu Yitong group (P<0.01); Compared with regular acupoits applying group, Shuanggu group, Yitong group, BMC and BMD all increased in Shuanggu Yitong group (P<0.05); There are no statistic significance among regular acupoits applying group, Shuanggu group, Yitong group (P>0.05). Conclusion: Acupuncture can increase BMC and BMD of osteoporosis model rats without ovaries and "Shuanggu Yitong" acupuncture therapy superior to other regular acupoints applying acupuncture therapy.(3) The indexes of E2 measurement indicate: Compared with control group, in the other four groups, E2 all increased (P<0.05), E2 increased significantly in Shuanggu Yitong group (P<0.01); Compared with regular acupoits applying group, Shuanggu group, Yitong group, E2 increased in Shuanggu Yitong group (P<0.05); There are no statistic significance among regular acupoits applying group, Shuanggu group, Yitong group (P>0.05). Conclusion: Acupunctur...