| Objective Hypothyroidism is a common disease of endocrine system, which could to lead to pathological changes and syndromes of many systems. It is common in clinical that hypothyroidism merging the impairment of brain and with the development of hypothyroidism, every patient have the impairment of brain, showing neurotic and psychiatric symptoms. Now only thyroxine sodium is used in treatment of hypothyroidism itself and has no specially therapeutic methods for the impairment of brain caused by hypothyroidism in clinic, and the effect is dissatisfaction. So it is therapeutic current with Chinese medicine for prevention and treatment of the impairment of brain caused by hypothyroidism by giving full play to the advantages of Traditional Chinese Medicine. It was confirmed that the therapeutic effect of Wenshentang in clinic is well. It could improve the symptoms not only hypothyroidism itself but also the impairment of brain caused by hypothyroidism. We study the function of Wenshentang on the impairment of brain caused by hypothyroidism, and its mechanism under the basic theory and experimental methods of modern medicine with the hypothyroidism animal model in order to provide a theory and experimental base for the clinical practice.Methods The rats were randomly divided into 5 groups, such as normal control group, model group, thyroxin group, small dosage group of Wenshentang and the large dosage of Wenshentang. The rats of the latter 4 groups were made model by infusing 0.04% tapazole suspension 1.35%/200g/day for 45 days. After that, the rats were treated with corresponding medicines for 15 days. Then radioimmunoassay method wasused on serum FT3, FT4, T3, T4 and TSH; Water labyrinth and shuttling box methods were used on the behavior changes; TUNEL methods was used on the cell apoptosis, RT-PCR method was used on the expression of bcl-2 in the nerve cell of hypothyroidism rats.Results (1)In the rats of model group, the behavior changes of kidney yang-insufficiency in hypothyroidism were appeared, and the indexes which may imply the severe degree of hypothyroidism presented the decreasing of FT3, FT4 and the increasing of TSH compared with the normal control group. Wenshentang, especially with large dosage had not only improved the symptoms of hypothyroidism, but also increased the content of FT3, FT4 and decreased the content of TSH, but the effect was not better than that of thyroxin group. (2) In the rats of model group, there have the decreasing of FT3, FT4and the increasing of TSH compared with the normal control group. The large dosage of Wenshentang and thyroxin could significantly improve those changes and the small dosage of Wenshentang could significantly only improve the change of FT4. (3) Compared with the normal control group, the Golgi body increase and fat, mitochondrion decrease or out of shape or bubbled, apoptosis corpuscle formed, smooth endoplasmic reticulum expand or increase in the ultrastructural of nerve cells of the hypothyroidsm rats. After treatment with Wenshentang, cell starch bubbled, mitochondrion decrease and the structure was basic normal and in small dosage cell ground substance decrease slightly, the structure was basic normal. (4) Compared with the normal control group, the count of apoptosis nerve cells of the hypothyroidsm rats was increase significantly and it was decrease significantly after treatment with Wenshentang. (5) Compared with the normal control group, the expression of bcl-2 was decrease significantly in nerve cells ofthe hypothyroidsm rats. It was increase significantly after treatment with Wenshentang and the more the dose the more the effect.Conclusion Wenshentang significantly improve the behavior disorder of the hypothyroidsm rats, increase the content of FT3, FT4 and decrease the content of TSH, improve the ultrastructural of nerve cells, decrease the count of apoptosis nerve cells, increase the expression of bcl-2.Those implied that Wenshentang could improve the brain impairment caused by Hypothyroidism significantly. |
PDF Full Text Request