| Objective Hypothyroidism is a common disease of endocrine system, which could to lead to pathological changes and syndromes of many systems. It is common in clinical that hypothyroidism merging the impairment of brain and with the development of hypothyroidism, every patient have the impairment of brain, showing neurotic and psychiatric symptoms. Now only thyroxine sodium is used in treatment of hypothyroidism itself and has no specially therapeutic methods for the impairment of brain caused by hypothyroidism in clinic, and the effect is dissatisfaction. So it is therapeutic current with Chinese medicine for prevention and treatment of the impairment of brain caused by hypothyroidism by giving full play to the advantages of Traditional Chinese Medicine. It was confirmed that the therapeutic effect of Wenshentang in clinic is well. It could improve the symptoms not only hypothyroidism itself but also the impairment of brain caused by hypothyroidism. We study the function of Wenshentang on the impairment of brain caused by hypothyroidism, and its mechanism under the basic theory and experimental methods of modern medicine with the hypothyroidism animal model in order to provide a theory and experimental base for the clinical practice.Methods The rats were randomly divided into 5 groups, such as normal control group, model group, thyroxin group, small dosage group of Wenshentang and the large dosage of Wenshentang. The rats of the latter 4 groups were made model by infusing 0.04% tapazole suspension 1.35%/200g/day for 45 days. After that, the rats were treated with corresponding medicines for 15 days. Then the following detection were taken out: radioimmunoassay on serum FT3, FT4, T3, T4 and TSH; water labyrinth and shuttling box methods on the behavior changes; RT-PCR, Western blot, Lowery, improved Takai and immunohistochemical method on the content or expression of NOmRNA, Go a mRNA and the activity of protein kinase C (PKC) in the hippocampus of hypothyroidism rats.Results (1) In the rats of model group, the behavior changes of kidney yang-insuf f iciency in hypothyroidism were appeared, and the indexes which may imply the severe degree of hypothyroidism presented the decreasing of FT3, FT4, T3, T4 and the increasing of TSH compared with the normal control group. Wenshentang, especially with large dosage had not only improved the symptoms of hypothyroidism, but also increased the content of FT3, FT4, T3, T4 and decreased the content of TSH, but the effect was not better than that of thyroxin group. (2) Wenshentang significantly improved the behavior disorder of the hypothyroidsm rats. The evasive latent period and length of swimming were significantlyshorten in the experiment of Morris water labyrinth, at the same time the times and the hours of get electric shock were significantly decreased in the experiment of shuttling box. The effect of large dosage group was better than that of thyroxin group. (3) Wenshentang significantly increased the content or expression of NOmRNA, decreased the content or expression of Go a mRNA and downregulation the activity of PKC of the hypothyroidsm rats, and the effect was better than that of thyroxin group.Conclusion In the rats of model group, the behavior changes of kidney yang-insufficiency in hypothyroidism were appeared , and the indexes which may imply the severe degree of hypothyroidism presented the decreasing of FT3, FT4, T3, T4 and the increasing of TSH compared with the normal control group. Wenshentang had not only improved the symptoms of hypothyroidism rats, but also increased the content of FT3, FT4, T3, T4 and decreased the content of TSH, but the effect were not excel the thyroxin group. Wenshentang significantly increased the content or expression of NOmRNA, decreased the content or expression of Go a mRNA and down regulation the activity of PKC of the hypothyroidism rats, and the effect were excel the thyroxin group. Wenshentang significantly improved the behavior disorder of the hypothyroidism rats which implied that Wenshentang have signific... |
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