Therapy Of Peri-implantitis In Dogs With Low Temperature Atmospheric Pressure Plasma And Scaling

Objective:To evaluate the clinical, microbiological radiological, and histological effects with low temperature atmospheric pressure plasma and scaling against the ligature-induced peri-implantitis in dogs.Material and Methods:Ligature-induced peri-implantitis was performed for six beagle dogs at 3 months after dental implants. When approximately 40% of the supporting bone was lost, the ligatures were removed. Scaling and local antiseptic cleansing using 0.2% chlorhexidine digluconate were performed in control group. Scaling, local antiseptic cleansing using 0.2% chlorhexidine digluconate and low temperature atmospheric pressure plasma were performed in experimental group. Clinical and radiographic examinations were performed before and at 3 months after treatment respectively. Micro CT and histological examinations were performed at 3 months after treatment. Microbiological samples were obtained and analysed at basement and 1 month,2 moths, and 3 months after treatment respectively.Results:The prevalence of Pg and Bf was significantly reduced after treatment. Sulcus Bleeding Index (SBI) and probing depth (PD) have significantly decreased (P< 0.05) from 4.42±0.58 and 4.77±1.45 mm to 1.21±0.58 and 2.52±0.7 mm respectively in low temperature atmospheric pressure plasma and scaling group. The differences between the experimental group and the control group were statistically significant (P< 0.05) after treatment in SBI and PD. The radiologically determined distance from the shoulder of the implant to the bottom of the bony defect (DSB) in experimental group significantly decreased from 3.35±0.70 mm to 2.21±0.90 mm (P < 0.05). The DSB in control group decreased from 3.27±0.90 mm to 2.88±0.90 mm and the decrease was not statistically significant. The differences of DSB between the experimental group and the control group after treatment were statistically significant (P< 0.05). The experimental group presented a higher bone height gain. Re-osseointegration occurred in the experimental group.Conclusions:Therapy of peri-implantitis in dogs with low temperature atmospheric pressure plasma and scaling had positive effects on clinical, microbiological, radiologically and histological parameters.