The Demonstration Of The Theory Of Nonenzymatic Glycation Inducing Aging

Gerontological studies performed at the organismic, cellular, and molecular levels have generated numerous theories that attempt to explain the events leading to biological senescence. In general, theories of aging have been either programmatic, postulating that aging is due to an inherent genetic programmatic, or stochastic, suggesting that aging results from environment damage. Now many studies have focused on the theory of nonenzymatic glycation inducing aging. It has been proposed that elevated level of AGE in vivo may accelerate aging process in animals and humans. Indeed, the AGE formation inhibitor, aminoguanidine, could prevent certain age-associated changes in rat aging model. However, direct evidence supporting that glycation is involved in the pathogenesis of aging is still limited. To investigate the role of AGE in aging, 3-month-old C57 mice were injected daily with D-galactose, D-galactose modified lysine (AGE-lysine), L-glucose, L-lysine, or control buffer for 60 days. Two additional groups were treated with an AGE formation inhibitor, aminoguanidine, and one was also injected daily with D-galactose.Within the period of treatment, all groups of mice gained weight normally. Serum AGE levels were determined by AGE-ELISA. As anticipated, old mice treated with PBS had an higher level of serum AGEs than that of young mice (P < 0.01). Young mice treated with D-galactose, L-glucose, AGE-lysine showed an increased level of serum AGEs comparing with young control mice (P < 0.05). AG, however, could prevent AGE increase in D-galactose treated mice. L-lysine or AG alone did not alter serum AGE levels in young mice.Neuromuscular movement was determined by spontaneous motor activity. As a common change associated with aging, old mice showed significant lower activity in 10 min than young mice (P < 0.05). D-galactose, L-glucose, AGE-lysine treatment significantly lowered the spontaneous motor activity of young mice comparing with controls (P < 0.01 or 0.05). The effect of D-galactose was...