Experimental Study For Anaphylactic Shock Models And Mechanisms In Sheep Induced By Echinococcus Granulosus

Experimental Study on Anaphylaxis induced by Echinococcus Infection in Meriones Unguiculatus Abstract Objective This study was to induce anaphylaxis in Meriones unguiculatus (gerbils) infected by Echinococcus.granulosus or Echinococcus. multi/ocularis (E.g. and F.m.) meanwhile, to observe the change of lymphatic microcirculation with electron microscopy. Methods 56 and 48 gerbils were intraperitoneally inoculated by E.g. protoscoleces and E.m. tissues homogenate. After three months, anaphylactic responses were initiated by intra- peritoneal challenging injection with E.g. cystic fluids and partially purified antigen or F.m. metacestode homogenate antigen . IgE antibody level and eosinophilic leukocyte numbers were detected by ELISA and DELC between pre-postchallenge. Meantime, in the reversible and irreversible shock gerbils ,we removed lymph gland of th gerbils for observing the change by eiectro- microscopy. Results The infection rates was 89.3% for E.g. and 97.9% for E.m. . Among those groups, anaphylaxis between 62.5% and 100%, anaphylactic shock between 12.5% and 16.7%. After infection with E.g. and E.m. in gerbils, both tgE antibody levels and DELC showed significantly increased. At 60 minutes post challenge, IgE antibody level were significantly decreased, in contrast, DELC showed a sharp increase. Ultrastructure of endothelial cells showed serious injuring in both irreversible and reversible shocked gerbils. Conclution Anaphylaxis could be induced in gerbils with Echinococcus infection material by challenging injection. Different antigens lead to different incidences and degrees those gerbils with anaphylaxis responses . However there were no significant differences between E.g. and E.m. infections. The function of lymphatic microcirculation plays an important role in the development and overcome of anaphylactic shock.