Studies On Isolation, Purification, Structural And Functional Character Of Vitis L. Polysaccharides

In recent years, some clinic-valuable polysaccharide and its derivative biochemical medicines have been found. It has already successively reported the methods about the extractionN isolation identification and the achievement.The study use Xinjiang currant as rough material, using polysaccharide abstraction depuration technique, instrumental analysis technique, race hygiene conservancy food function evaluate method, modern medicine analysis technique and so on, having done some deep systemic research on the extraction, isolation, identification, purification of VLP and the components and constructions features, then estimating the immunity function , repel tumor and decrease blood sugar research of VLP rough production, which give it the foundation for the further research. The researches about grape polysaccharides have not been reported.1 Through the research of VLP abstraction and purification, we found that VLP can be got by water extracting and alcohol precipitating, chloroform degreasing, eliminating albumen with the method of Sevag, dialysis and freeze drying from raisin, the rate is 8.68%. we got four fractions through the VLP Sephadex A-25 chromatography, it shows that the VLP of the four fractions are homogeneous after paper chromatography identification. It also shows that the four fractions are all homogeneous polysaccharides through Sephadex G-200 arbitration, acrylamide gel electrophoresis arbitration. Calculated with the rough polysaccharide as initiative material, the polysaccharide rates of the four fractions are as follows: VLP -1 is 25.32%,VLP-2 is 50.82%,VLP-3 is 7.55%,VLP-4 is 10.41%.According to Gas Chromatography infra-red spectra analysis, the four fractions all contain different quantities of arabinose , galactose, glucose and seminose, VLP-2 and VLP-3 also contain rhamnose and xylose, polysaccharide configuration may have B -D-pyran glucose.VLP-1 , VLP-2 contain abundant amino acid, such as Phe, Asp Arg and Thr. The analysis of 1H-NMR and 13C-NMR also infect the same results above.2 The study of the immunity function of VLPImmunity is a protective physiological reaction to the body, which has the knowing or respond ability to the self and others antigen. The existence of the immunity system and its functional normalization are the elemental guaranty of the body immunity function. Immunize adjusting function is the most important function of activity polysaccharide, which has a tight relationship with the function of repel tumor, oxidation prevention,decreasing blood sugar and adepts of polysaccharide. The immunization adjusting function is actually immunization respond caused by polysaccharide as antigen. The result of the experiment shows , polysaccharide dosage groups have apparent promoter actions to the transition of normal rats' achroacyte. Among them VLP- 1 100 mg / kg d acts best, the transition rate of achroacyte reach 65.5013%, each VLP -1 dosage has apparent effects to the live weight gain of normal rats' immunization organic, VLP-1 50 mg / kg d and 100 mg / kg d have sorely significantly live weight gain function to thymus and milt, when VLP-1 is 25 mg / kg d, the thymus weight increase sorely significantly while milt increase not too much. Except that 100 mg / kg d rough polysaccharide has apparently effects to the increase of milt, other dosages has no effects to normal rats' thymus and milt, VLP has apparent facilitation to DTH. When rough polysaccharide is 50 mg / kg d its function is the most obvious, next is 25 mg / kg d; all dosages for immunity organs have substantial weights increase. VLP can prompt apparently the phagocytosis function of macrophage cells.From above we can see that VLP is able to increase all aspects of immunity function of cells and humors, increase the weights of immunity organs.3 The research of VLP repelling tumors.The fundamental object of tumor research is reducing the lethality and morbidity, reducing the lethality mainly depends on treatment, reducing morbidity hinges on prophylaxis. Tumors...