| Objective:To explore the effects of different exercise modes on proteomic characteristics of subcutaneous adipose tissue in high-fat diet rats based on TMT proteomics technology.Methods:48 3-week-old male SD rats were randomly divided into standard quiet group(C),standard moderate-intensity continuous training group(CE),standard high-intensity interval training group(CH),high-fat quiet group(HS),high-fat moderate-intensity continuous training group(HE),high-fat high-intensity interval training group(HH)after one week of adaptive feeding,each of eight.Rats of the C,CE and CH groups were fed normal diet,while those in the HC,HE and HH groups were fed high-fat diet.The training group received the exercise intervention for 8 weeks,5 days a week.Rats of the CE and HE groups underwent 60%-70%VO2max intensity continuous treadmill training for 50 minutes.Before and after each training,CH group and HH group were given 7 minutes of warm-up and recovery treadmill training at 70%VO2max intensity respectively,followed by high-intensity interval training(3min90%VO2max+3min50%VO2max)×6 times,for a total of 50min.After the intervention,the body weight of the rats was weighed,the body composition of the rats was measured by DEXA scanning,and the subcutaneous adipose tissue was taken for wet weight measurement.Three rats from each group who completed the intervention process were randomly selected for subcutaneous adipose tissue TMT proteomics test.Differential proteins were screened and bioinformatics analysis was performed.Results:(1)Training significantly decreased the body weight of rats in the standard diet group(P<0.05),and at the same time significantly decreased the body fat percentage of rats in the high fat diet group(P<0.01).(2)The results of proteomic functional enrichment showed that in the standard diet group,with the C group as the control,there were significant differences in 285 and 478 proteins between the CE group and the CH group.Bioinformatics showed that differential proteins were related to RNA polymerase and Toll-like receptor signaling pathway and so on.In the high-fat diet group,with the HC group as the control,there were significant differences in 38 and 164 proteins between the HE group and the HH group respectively.Bioinformatics showed that differential proteins were related to RNA polymerase and NOD-like receptor signaling pathway and so on.Under the same exercise state or exercise modes,65,175,406 proteins were significantly different between HC group and C group,HE group and CE group,and HH group and CH group,respectively.Bioinformatics showed that the differential proteins were related to influenza A,hepatitis C and other disease pathways.(3)The results of protein PPI interaction showed that in the standard diet group,the key proteins of PPI network of different exercise modes were related to RNA polymerase and ribosomal protein,etc.In the high-fat diet group,the key proteins of PPI network in different exercise modes were related to mitochondrial fission protein and ribosomal protein,etc.Under the same exercise state or exercise mode,the key proteins of PPI network in different diet were related to lipoprotein and mitochondrial protein,etc.Conclusion:(1)In the standard diet group,both MICT and HIIT could affect the immune response and molecular transcription process in the protein expression profile of subcutaneous adipose tissue of rats,but their respective influence immune pathways were different.In MICT,mainly downstream changes of Toll-like receptor signaling pathways were found,while HIIT mainly included changes in JAK-STAT signaling pathways.A total of 30 hub genes in PPI network,including Polr2c,Polr2e,Rpl17,Apoa1 and Ndufb7,were screened out for subsequent mechanism study.(2)In the highfat diet group,MICT and HIIT can also affect the immune response mechanism in the protein expression profile of subcutaneous adipose tissue of rats,but their respective influencing pathways are different,and the anti-inflammatory effect of MICT is more obvious.The two kinds of locomotion modes had obvious influence on the molecular transcription process.A total of 22 hub genes in PPI networks,such as Apaf1,Fis1,Mrps7 and Gart,were screened out for further mechanism study.(3)Under the same exercise intervention,no matter in the quiet state or with two exercise interventions,the choice of diet will affect the biomolecular processes of adipose tissue.Even if exercise intervention is carried out in the long-term high-fat diet,the metabolic process and some immune processes of adipose tissue will also be affected.In addition,a total of 30 hub genes in the PPI network,including Apoal,Apoa2 and Mrpl4,were screened out for subsequent mechanism study. |
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