Objective:To establish a guinea pig model of form deprivation deficiency of qi and blood in myopia,observe the effect of Chinese herbal medicine Angelica sinensis on choroidal thickness and its preventive and therapeutic effects on myopia in guinea pigs with form deprivation deficiency of qi and blood.Materials and methods:Forty healthy tricolor guinea pigs were randomly divided into blank control group,model control group,positive control group,high dose group and low dose group of angelica sinensis.Except the blank control group,the models of monocular myopia and deficiency of qi and blood in traditional Chinese medicine were established in the other four groups.The experiment period was 8 weeks.The blank control group was fed normally without any intervention.The model control group was not given the drug after modeling treatment.The positive control group was given 0.01% atropine sulfate eye drops as drug control;The high-dose and low-dose groups of angelica sinensis were treated by gavage with different concentrations of angelica intelligent granules.The intraocular pressure,diopter,eye axis and pupil diameter of all guinea pigs were measured at regular intervals.OCT scanning images of guinea pig fundus were collected and choroidal thickness was measured.At the end of the experiment,all guinea pigs were sacrificed,and their right eyes were stained with HE to observe the morphology and thickness of choroid and sclera tissue.Tunel staining was performed to detect the apoptosis of retina,choroid and sclera.The expressions of MMP-2 and HIF-1α in guinea pig sclera were detected by immunofluorescence method.Results:1.On the 56 th day of the experiment,guinea pigs in model control group,positive control group,high-dose and low-dose groups of angelica sinensis all developed relative myopia,and their general state decreased to different degrees compared with blank control group.2.On day 56 of the experiment,the diopter value of guinea pigs in the model control group was significantly higher than that in the blank control group(P<0.001).Compared with model control group,the change of diopter value of guinea pigs in positive control group and angelica high-dose group was significantly decreased(P<0.001);The change of diopter value in angelica low-dose group was slightly decreased(P<0.05).Compared with blank control group,the axial length of guinea pigs in model control group was significantly increased(P<0.001).Compared with model control group,axial length of guinea pigs in positive control group and high dose group of angelica sinensis was significantly decreased(P<0.01).On the56 th day of the experiment,there was no significant difference in intraocular pressure among all groups(P>0.05).3.OCT scanning of fundus: on day 56 of the experiment,the choroid thickness of guinea pigs in the model control group was significantly thinner than that in the blank control group(P<0.01).Compared with model control group,the choroid thickness of guinea pigs in positive control group and angelica sinensis high-dose group showed an increasing trend(P<0.05),but there was no statistical significance between angelica sinensis low-dose group and model control group(P>0.05).4.HE staining: At the end of the experiment,compared with the blank control group,the choroid thickness of the model control group was slightly thinner(P<0.05),and the sclera thickness was significantly thinner(P<0.001).Compared with model control group,choroid thickness and sclera thickness of angelica high-dose group were significantly increased(P<0.001);The choroid thickness of angelica sinensis low-dose group was increased(P<0.05).5.Tunel staining: At the end of the experiment,the number of apoptotic cells in retina,choroid and sclera in model control group was significantly increased(P<0.001).Compared with model control group,the number of apoptosis of retina and choroid cells in positive control group,high-dose and low-dose angelica groups was significantly decreased(P<0.001).The number of scleral cell apoptosis in high-dose angelica group was significantly decreased compared with model control group(P<0.001).Positive control group and angelica low-dose group were followed(P<0.01).6.Immunofluorescence staining: At the end of the experiment,the expression of MMP-2 in model control group was significantly increased(P<0.001).The expression of MMP-2 in sclera of positive control group and high-dose angelica sinensis group was significantly decreased compared with model control group(P<0.001).At the end of the experiment,the expression of HIF-1α in model control group was significantly increased(P<0.01).The expression of HIF-1α in sclera of positive control group and angelica high-dose group was significantly decreased(P<0.01).Conclusion:1.Guinea pigs were treated with monocular form deprivation combined with restricted diet and bilateral ear edge incision bloodletting to establish a form deprivation myopia model of deficient qi and blood.With the extension of molding time,the diopter value of myopia gradually deepened and the axis of eye gradually lengthened.2.Angelica sinensis can inhibit the increase of diopter and eye axis in guinea pigs with form deprivation deficiency of qi and blood myopia and has a preventive and therapeutic effect on form deprivation deficiency of qi and blood myopia.3.Angelica sinensis,as a blood-tonifying and blood-activating drug,can increase choroidal thickness,reduce scleral HIF-1α and MMP-2 expression in the posterior pole of eyeball of myopic guinea pigs,relieve the state of ischemia and hypoxia in myopic eyes,inhibit scleral remodeling,and thus interfere with the development of myopic hypoglycemic myopia.4.Angelica sinensis can inhibit the apoptosis of retina,choroid and sclera tissue in the posterior pole of eyeball in myopic guinea pig,suggesting that Angelica sinensis can control the development of myopia,which may be related to the inhibition of apoptosis in the posterior pole of eyeball tissue of myopic guinea pig. |