| Objective:This research sought to explore the correlation between apoptosis and RSA,utilizing the Caspase-3/Bax pathway.The aim was to assess the potential impact of Shoutai pill on Intervening the occurrence of RSA by controlling apoptosis through the Caspase-3/B ax pathway,and to provide a clinical basis for the utilization of Shoutai pill for the treatment of RSA.Methods:This experiment selected Specified Pathogen Free(SPF)rats as the experimental subjects,including 48 female rats aged 8 weeks without mating,with weight of 218.70±9.09g;24 male rats aged 10 weeks without mating,with weight of 243.5 5±9.6 8g;After one week of standardized breeding,mating was carried out overnight in a ratio of 2:1 between females and males,with a large amount of sperm or vaginal plugs found on vaginal smears as the criteria for successful mating.48 female rats were randomly divided into four groups:the normal control,the model,the dydrogesterone,and the Shoutai pill group,with 12 in each.Using the human-rat body surface area conversion method,the dose of adult rats and the dose of rehmanniacin were converted.From the day of pregnancy,every morning,the Shoutai Wan group took 12 g/kg Shoutai Wan liquid orally,and the Dydrogesterone group took 4.55 mg/kg Dy drogesterone water for oral administration.Gastrointestinal administration(according to the effective dose of 60 kg for adults is 60 kg),0.9%sodium chloride aqueous solution was used as the model group and the normal control group.Every afternoon:on the basis of 0.9%NS,continue intragastric administration,and prepare rat models of Hydroxyurea(4 5 0 mg/kg)on the basis of 0.9NS.On the 10th day of gestation,4.0 mg/kg mifepristone was administered in a single dose to each group,and their food intake,water consumption and body weight were documented.Subsequently,intragastric administration was used to create the rat RSA animal model.On the 10th day,one hour after administration,the rats were sacrificed by cutting their necks.All the uterine tissues including decidua of rats in each group were excised and preserved.Calculate the embryonic loss rate of rats in each group.Western blotting technique was utilized to analyze Caspase-3 and Bax in rat decidua.Quantitative analysis of Caspase-3 and Bax in rat decidua was carried out by fluorescent quantitative PCR technology.Results:The blank group showed a much lower expression of Bax and Caspase-3 proteins than the model group(**P<0.01).The didravone group,however,exhibited significantly lower expression(##P<0.01,#P<0.05);and,in contrast to the model group,the Shoutai Pill group showed a significant decrease in Bax and Caspase-3 protein expression(##P<0.01,#P<0.05).The model group’s relative expression of Bax and Caspase-3 mRNA was significantly higher than the control group’s,with a p-value of less than 0.01,in comparison to the blank group.A marked contrast was observed between the two groups;the didroxyprogesterone group exhibited significantly diminished Bax and Caspase-3 mRNA expression in comparison to the model group(##P<0.01);and the Shoutai pill group’s decidual tissue expression of Bax and Caspase-3 mRNA was significantly lower than that of the model group(##P<0.01).Conclusion:The classical Caspase-3/Bax pathway may be the possible reason of Apoptosis,which could be the cause of RSA.Shoutai pill may play an important role in embryo preservation by inhibiting Caspase-3/Bax-mediated apoptosis pathway. |
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